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Literature summary for 3.1.1.20 extracted from

  • Cavalcanti, R.M.F.; Jorge, J.A.; Guimaraes, L.H.S.
    Characterization of Aspergillus fumigatus CAS-21 tannase with potential for propyl gallate synthesis and treatment of tannery effluent from leather industry (2018), 3 Biotech, 8, 270 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.20

Activating Compound

Activating Compound Comment Organism Structure
KH2PO4 slightly Aspergillus fumigatus
methanol at 1% v/v Aspergillus fumigatus
SDS at 1% w/v Aspergillus fumigatus
Triton-X100
-
 Aspergillus fumigatus
Urea about 1.8fold at 4 mM, no effect at 1-3 M Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol at 1 mM Aspergillus fumigatus
acetone at 1% v/v Aspergillus fumigatus
acetonitrile at 1% v/v Aspergillus fumigatus
Ba2+
-
 Aspergillus fumigatus
butanol at 1% v/v Aspergillus fumigatus
Co2+
-
 Aspergillus fumigatus
CuSO4
-
 Aspergillus fumigatus
EDTA at 1 mM Aspergillus fumigatus
ethanol at 1% v/v Aspergillus fumigatus
Fe2+ at 10 mM Aspergillus fumigatus
Fe3+ at 10 mM Aspergillus fumigatus
Isopropanol at 1% v/v Aspergillus fumigatus
MnCl2
-
 Aspergillus fumigatus
additional information no or poor inhibition by Ca2+, CuCl2, Hg2+, Mg2+, and Zn2+ Aspergillus fumigatus
NaCl
-
 Aspergillus fumigatus
NH4Cl
-
 Aspergillus fumigatus
Tween-20 at 0.01% v/v Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.61
-
 propyl gallate pH 5.0, 35°C Aspergillus fumigatus
6.28
-
 methyl gallate pH 5.0, 35°C Aspergillus fumigatus
6.38
-
 Tannic acid pH 5.0, 35°C Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus fumigatus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
AgNO3 activates Aspergillus fumigatus
MnSO4 activates Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60340
-
 gel filtration Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-
Aspergillus fumigatus CAS-21
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme is a glycoprotein with 39.1% carbohydrate content Aspergillus fumigatus

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 4.9fold from submerged fermentation broth by anion exchange chromatography, dialysis, and gel filtration, followed by another step of dialysis Aspergillus fumigatus

Source Tissue

Source Tissue Comment Organism Textmining
submerged culture
-
 Aspergillus fumigatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl gallate + H2O
-
 Aspergillus fumigatus methanol + gallic acid
-
 ?
methyl gallate + H2O
-
 Aspergillus fumigatus CAS-21 methanol + gallic acid
-
 ?
additional information colorimetric actiivty analysis using methanolic rhodanine, spectrometric quantification Aspergillus fumigatus ?
-
 ?
additional information colorimetric actiivty analysis using methanolic rhodanine, spectrometric quantification Aspergillus fumigatus CAS-21 ?
-
 ?
propyl gallate + H2O best substrate Aspergillus fumigatus propanol + gallic acid
-
 ?
propyl gallate + H2O best substrate Aspergillus fumigatus CAS-21 propanol + gallic acid
-
 ?
tannic acid + 1-propanol transesterification Aspergillus fumigatus gallic acid + propyl gallate
-
 ?
tannic acid + 1-propanol transesterification Aspergillus fumigatus CAS-21 gallic acid + propyl gallate
-
 ?
tannic acid + H2O
-
 Aspergillus fumigatus gallic acid + ?
-
 ?
tannic acid + H2O
-
 Aspergillus fumigatus CAS-21 gallic acid + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60385, SDS-PAGE Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
 Aspergillus fumigatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 over 80% of maximal activity within this range, profile overview Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 purified native enzyme, maintains 80% of its initial activity when incubated at 30°C for 60 min Aspergillus fumigatus
45 50 purified native enzyme, loses 50% activity after 120 min, the half-life is 60 min Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
111.93
-
 propyl gallate pH 5.0, 35°C Aspergillus fumigatus
152.86
-
 methyl gallate pH 5.0, 35°C Aspergillus fumigatus
362.24
-
 Tannic acid pH 5.0, 35°C Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 9 activity range, profile overview Aspergillus fumigatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 purified exracellular enzyme, 3 h, the enzyme is completely stable and retains 70% of its initial activity after 24 h Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
24.34
-
 methyl gallate pH 5.0, 35°C Aspergillus fumigatus
31
-
 propyl gallate pH 5.0, 35°C Aspergillus fumigatus
56.78
-
 Tannic acid pH 5.0, 35°C Aspergillus fumigatus