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Literature summary for 3.1.1.20 extracted from
- Biochemical characterization of Aspergillus oryzae native tannase and the recombinant enzyme expressed in Pichia pastoris (2014), J. Biosci. Bioeng., 118, 392-395.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain RIB40 | Aspergillus oryzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
epicatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae |  |
| 0.26 | - |
epigallocatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 0.32 | - |
epigallocatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 0.34 | - |
epicatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 0.45 | - |
catechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 0.47 | - |
catechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 0.86 | - |
ethyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 1.02 | - |
methyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 1.12 | - |
propyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 1.3 | - |
ethyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 1.38 | - |
propyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 1.97 | - |
methyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tannic acid + H2O | Aspergillus oryzae | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Aspergillus oryzae RIB 40 | - |
gallate + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
| Aspergillus oryzae RIB 40 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus oryzae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| TSKgel DEAE-5PW column chromatography and G3000-SWXL gel filtration | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechin gallate + H2O | - |
Aspergillus oryzae | gallate + catechin | - |
? | |
| epicatechin gallate + H2O | best substrate | Aspergillus oryzae | gallate + epicatechin | - |
? | |
| epicatechin gallate + H2O | best substrate | Aspergillus oryzae RIB 40 | gallate + epicatechin | - |
? | |
| epigallocatechin gallate + H2O | - |
Aspergillus oryzae | gallate + epigallocatechin | - |
? | |
| ethyl gallate + H2O | worst substrate | Aspergillus oryzae | gallate + ethanol | - |
? | |
| methyl gallate + H2O | - |
Aspergillus oryzae | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Aspergillus oryzae RIB 40 | gallate + methanol | - |
? | |
| additional information | no activities against the methyl esters of ferulic, 4-coumaric, caffeic, and sinapic acids, or the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid | Aspergillus oryzae | ? | - |
? | |
| additional information | no activities against the methyl esters of ferulic, 4-coumaric, caffeic, and sinapic acids, or the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid | Aspergillus oryzae RIB 40 | ? | - |
? | |
| propyl gallate + H2O | - |
Aspergillus oryzae | gallate + propanol | - |
? | |
| propyl gallate + H2O | - |
Aspergillus oryzae RIB 40 | gallate + propanol | - |
? | |
| tannic acid + H2O | - |
Aspergillus oryzae | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Aspergillus oryzae RIB 40 | gallate + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000-33000, deglycosylated native enzyme, SDS-PAGE | Aspergillus oryzae |
| ? | x * 30000-34000, deglycosylated recombinant enzyme, SDS-PAGE | Aspergillus oryzae |
| ? | x * 45000-75000, recombinant enzyme, smeared bands on SDS-PAGE | Aspergillus oryzae |
| ? | x * 45000-80000, native enzyme, smeared bands on SDS-PAGE | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TanA | - |
Aspergillus oryzae |
| tannin acyl hydrolase | - |
Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | the enzyme is stable up to 40°C. Native enzyme retains more than 70% of ist activity after incubation at 50°C for 60 min, whereas the residual activity of recombinant enzyme is approximately 40% after treatment at 50°C for 60 min | Aspergillus oryzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.8 | - |
ethyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 18.6 | - |
propyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 19 | - |
ethyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 20.5 | - |
propyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 60.2 | - |
methyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 62.1 | - |
epigallocatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 64.5 | - |
catechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 67.5 | - |
epicatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 70.2 | - |
methyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 79.8 | - |
catechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 86.1 | - |
epigallocatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 103.4 | - |
epicatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | native enzyme | Aspergillus oryzae |
| 5 | - |
purified enzyme | Aspergillus oryzae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.54 | - |
ethyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 14.9 | - |
propyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 16.6 | - |
propyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 22.1 | - |
ethyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 30.6 | - |
methyl gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 69.2 | - |
methyl gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 137.3 | - |
catechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 179.3 | - |
catechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 234 | - |
epigallocatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 273.4 | - |
epigallocatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 293.6 | - |
epicatechin gallate | recombinant enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
| 308.8 | - |
epicatechin gallate | native enzyme, at pH 5.0 and 30°C | Aspergillus oryzae | |
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Release 2023.1 (January 2023)
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