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Literature summary for 3.1.1.20 extracted from

  • Jimenez, N.; Esteban-Torres, M.; Mancheno, J.M.; de Las Rivas, B.; Munoz, R.
    Tannin degradation by a novel tannase enzyme present in some Lactobacillus plantarum strains (2014), Appl. Environ. Microbiol., 80, 2991-2997.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.20

Activating Compound

Activating Compound Comment Organism Structure
DMSO about 130% activity at 1 mM Lactiplantibacillus plantarum
Triton X-100 about 150% activity at 1 mM Lactiplantibacillus plantarum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lactiplantibacillus plantarum

Inhibitors

Inhibitors Comment Organism Structure
beta-mercaptoethanol about 10% residual activity at 1 mM Lactiplantibacillus plantarum
EDTA about 30% residual activity at 1 mM Lactiplantibacillus plantarum
Hg2+ complete inhibition at 1 mM Lactiplantibacillus plantarum
Zn2+ about 65% residual activity at 1 mM Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 175% activity at 1 mM Lactiplantibacillus plantarum
Mg2+ about 120% activity at 1 mM Lactiplantibacillus plantarum
additional information not affected by K+ Lactiplantibacillus plantarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 x * 67000, SDS-PAGE Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Lactiplantibacillus plantarum
-
 gallate + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum ATCC 14917T
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butyl 4-hydroxybenzoate + H2O
-
 Lactiplantibacillus plantarum butanol + 4-hydroxybenzoate
-
 ?
epigallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum epigallocatechin + gallate
-
 ?
ethyl 3,4-dihydroxybenzoate + H2O
-
 Lactiplantibacillus plantarum ethanol + 3,4-dihydroxybenzoate
-
 ?
ethyl 3,4-dihydroxybenzoate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T ethanol + 3,4-dihydroxybenzoate
-
 ?
ethyl 3,5-dihydroxybenzoate + H2O
-
 Lactiplantibacillus plantarum ethanol + 3,5-dihydroxybenzoate
-
 ?
ethyl 4-hydroxybenzoate + H2O
-
 Lactiplantibacillus plantarum ethanol + 4-hydroxybenzoate
-
 ?
ethyl 4-hydroxybenzoate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T ethanol + 4-hydroxybenzoate
-
 ?
ethyl benzoate + H2O
-
 Lactiplantibacillus plantarum ethanol + benzoate
-
 ?
ethyl benzoate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T ethanol + benzoate
-
 ?
ethyl gallate + H2O
-
 Lactiplantibacillus plantarum gallate + ethanol
-
 ?
ferulic ethyl ester + H2O
-
 Lactiplantibacillus plantarum ethanol + ferulate
-
 ?
ferulic methyl ester + H2O
-
 Lactiplantibacillus plantarum methanol + ferulate
-
 ?
lauryl gallate + H2O
-
 Lactiplantibacillus plantarum gallate + lauric acid
-
 ?
methyl 2,4-dihydroxybenzoate + H2O
-
 Lactiplantibacillus plantarum methanol + 2,4-dihydroxybenzoate
-
 ?
methyl 4-hydroxybenzoate + H2O
-
 Lactiplantibacillus plantarum methanol + 4-hydroxybenzoate
-
 ?
methyl benzoate + H2O
-
 Lactiplantibacillus plantarum methanol + benzoate
-
 ?
methyl benzoate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T methanol + benzoate
-
 ?
methyl gallate + H2O
-
 Lactiplantibacillus plantarum gallate + methanol
-
 ?
methyl gentisate + H2O
-
 Lactiplantibacillus plantarum methanol + gentisate
-
 ?
methyl salicylate + H2O
-
 Lactiplantibacillus plantarum methanol + salicylate
-
 ?
methyl vanillate + H2O
-
 Lactiplantibacillus plantarum methanol + vanillate
-
 ?
methyl vanillate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T methanol + vanillate
-
 ?
propyl 4-hydroxybenzoate + H2O
-
 Lactiplantibacillus plantarum propanol + 4-hydroxybenzoate
-
 ?
propyl gallate + H2O
-
 Lactiplantibacillus plantarum gallate + propanol
-
 ?
protocatechuic acid ethyl ester + H2O
-
 Lactiplantibacillus plantarum ethanol + protocatechuate
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus plantarum gallate + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Lactiplantibacillus plantarum

Synonyms

Synonyms Comment Organism
TanA
-
 Lactiplantibacillus plantarum
tannin acyl hydrolase
-
 Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Lactiplantibacillus plantarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 60 more than 50% activity between 4 and 60°C Lactiplantibacillus plantarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22 65 the enzyme is quite unstable, losing about 50% of its activity after a 5 min incubation between 22 and 30°C, about 60% at 37°C, and is almost inactive after 5 min at 65°C Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Lactiplantibacillus plantarum

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 more than 50% activity between pH 4.5 and 8.0 Lactiplantibacillus plantarum

pI Value

Organism Comment pI Value Maximum pI Value
Lactiplantibacillus plantarum isoelectric focusing
-
 9.9

Expression

Organism Comment Expression
Lactiplantibacillus plantarum isoform TanA expression is not affected by methyl gallate additional information