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Literature summary for 3.1.1.20 extracted from

  • Wu, M.; Peng, X.; Wen, H.; Wang, Q.; Chen, Q.; McKinstry, W.J.; Ren, B.
    Expression, purification, crystallization and preliminary X-ray analysis of tannase from Lactobacillus plantarum (2013), Acta Crystallogr. Sect. F, 69, 456-459.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-AI cells Lactiplantibacillus plantarum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 14.6% (w/v) PEG 8000 in 0.1 M trisodium citrate pH 5.7 Lactiplantibacillus plantarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50777
-
 1 or 2 * 50777, the tannase exists as both a dimer and a monomer in solution, calculated from amino acid sequence Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
digallate + H2O Lactiplantibacillus plantarum
-
 gallate
-
 ?
tannic acid + H2O Lactiplantibacillus plantarum
-
 gallate + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum B3Y018
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity HisTrap column chromatography and Superdex 200 gel filtration Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
digallate + H2O
-
 Lactiplantibacillus plantarum gallate
-
 ?
epigallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum gallate + epigallocatechin
-
 ?
gallotannin + H2O
-
 Lactiplantibacillus plantarum gallate + D-glucose
-
 ?
methyl gallate + H2O
-
 Lactiplantibacillus plantarum gallate + methanol
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus plantarum gallate + D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer or dimer 1 or 2 * 50777, the tannase exists as both a dimer and a monomer in solution, calculated from amino acid sequence Lactiplantibacillus plantarum