Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
methyl gallate | at pH 8.0 | Lactiplantibacillus plantarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Lactiplantibacillus plantarum |
50700 | - |
deduced from the amino acid sequence | Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | B3Y018 | strain ATCC 14917(T) | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, hydroxylapatite column chromatography, and Mono-Q column chromatography | Lactiplantibacillus plantarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.5 | - |
crude extract, at pH 8.0 | Lactiplantibacillus plantarum |
84.34 | - |
after 11.25fold purification, at pH 8.0 | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl gallate + H2O | - |
Lactiplantibacillus plantarum | gallic acid + methanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Lactiplantibacillus plantarum |
Synonyms | Comment | Organism |
---|---|---|
TanLpl | - |
Lactiplantibacillus plantarum |
tannin acylhydrolase | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lactiplantibacillus plantarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | the enzyme retains more than 50% activity between 25 and 45°C but the activity drops off markedly above 45°C | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Lactiplantibacillus plantarum |