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Literature summary for 3.1.1.20 extracted from

  • Yu, X.; Li, Y.
    Expression of Aspergillus oryzae tannase in Pichia pastoris and its application in the synthesis of propyl gallate in organic solvent (2008), Food Technol. Biotechnol., 46, 80-85.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
1 * 33000 + 1 * 30000, purified tannase digested with N-glycosidase F and loaded with 2-mercaptoethanol, SDS-PAGE Aspergillus oryzae
33000
-
1 * 33000 + 1 * 30000, purified tannase digested with N-glycosidase F and loaded with 2-mercaptoethanol, SDS-PAGE Aspergillus oryzae
45000
-
purified tannase with 2-mercaptoethanol, SDS-PAGE Aspergillus oryzae
100000
-
purified tannase without 2-mercaptoethanol, SDS-PAGE Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae P78581
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propyl gallate + H2O
-
Aspergillus oryzae gallate + propanol
-
r

Subunits

Subunits Comment Organism
heterodimer 1 * 33000 + 1 * 30000, purified tannase digested with N-glycosidase F and loaded with 2-mercaptoethanol, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
tannin acyl hydrolase
-
Aspergillus oryzae