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Literature summary for 3.1.1.20 extracted from

  • Naidu, R.B.; Saisubramanian, N.; Selvakumar, D.; Janardhanan, S.; Puvanakrishnan, R.
    Partial purification of tannase from Aspergillus foetidus by aqueous two phase extraction and its characterization (2008), Curr. Trends Biotechnol. Pharm., 2, 201-207.
No PubMed abstract available
Search for more literature for 3.1.1.20

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ slight reduction in activity in the presence of Mg2+ Aspergillus foetidus
Zn2+ slight reduction in activity in the presence of Zn2+ Aspergillus foetidus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ highly stable in presence of 1 mM CaCl2 Aspergillus foetidus
Cu2+ highly stable in presence of 1 mM CuSO4 Aspergillus foetidus
Mn2+ highly stable in presence of 1 mM MnSO4 Aspergillus foetidus
Na+ highly stable in presence of 1 mM NaCl Aspergillus foetidus

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the enzyme shows considerable stability in the presence of commercial detergents such as Sunlight, Rinshakthi and Wheel Aspergillus foetidus
SDS the enzyme shows considerable stability in the presence of SDS Aspergillus foetidus
Triton X-100 considerable loss in activity in the presence of Triton X-100 Aspergillus foetidus
Tween 60 considerable loss in activity in the presence of Tween 60 Aspergillus foetidus

Organism

Organism UniProt Comment Textmining
Aspergillus foetidus
-
-
-
Aspergillus foetidus MTCC 6322
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by ammonium sulfate precipitation and aqueous two phase extraction with PEG6000 Aspergillus foetidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.7
-
 crude extract Aspergillus foetidus
88.3
-
 after 2.62fold purification Aspergillus foetidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tannic acid + H2O
-
 Aspergillus foetidus gallate + D-glucose
-
 ?
tannic acid + H2O
-
 Aspergillus foetidus MTCC 6322 gallate + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
tannin acyl hydrolase
-
 Aspergillus foetidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Aspergillus foetidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 40
-
 Aspergillus foetidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 significant decrease in thermal stability at temperatures above 40°C Aspergillus foetidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus foetidus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7
-
 Aspergillus foetidus