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Literature summary for 3.1.1.20 extracted from

  • Kasieczka-Burnecka, M.; Kuc, K.; Kalinowska, H.; Knap, M.; Turkiewicz, M.
    Purification and characterization of two cold-adapted extracellular tannin acyl hydrolases from an Antarctic strain Verticillium sp. P9 (2007), Appl. Microbiol. Biotechnol., 77, 77-89.
    View publication on PubMed
Search for more literature for 3.1.1.20

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol inhibits both isozymes TAH I and TAH II Hypocreales sp.
4-chloromercuribenzoate inhibits both isozymes TAH I and TAH II Hypocreales sp.
Ag+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
alpha-glutathione inhibits both isozymes TAH I and TAH II Hypocreales sp.
Cd2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
Co2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
CO32- inhibits both isozymes TAH I and TAH II Hypocreales sp.
Cu2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
Fe3+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
Hg2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
K+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
Mn2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
Pb2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
Sn2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
sodium dodecyl sulphate inhibits isozyme TAH II Hypocreales sp.
Triton X-100 inhibits both isozymes TAH I and TAH II Hypocreales sp.
Tween 20 inhibits both isozymes TAH I and TAH II Hypocreales sp.
Tween 60 inhibits both isozymes TAH I and TAH II Hypocreales sp.
Tween 80 inhibits both isozymes TAH I and TAH II Hypocreales sp.
Zn2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.05
-
 Tannic acid isozyme TAH I, pH 5.5, 20°C Hypocreales sp.
1.51
-
 Tannic acid isozyme TAH II, pH 5.5, 20°C Hypocreales sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Hypocreales sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Br- activates both isozymes TAH I and TAH II Hypocreales sp.
Mg2+ activates both isozymes TAH I and TAH II Hypocreales sp.
Urea activates both isozymes TAH I and TAH II Hypocreales sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE Hypocreales sp.
46000
-
 x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE Hypocreales sp.
155000
-
 isozymes TAH I and TAH II, gel filtration Hypocreales sp.

Organism

Organism UniProt Comment Textmining
Hypocreales sp.
-
 Antarctic strain P9, two cold-adapted isozymes TAH I and TAH II
-

Purification (Commentary)

Purification (Comment) Organism
native isozymes TAH I and TAH II 7.9fold and 10.5fold, respectively, to homogeneity Hypocreales sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
61.8
-
 purified isozyme TAH I Hypocreales sp.
82.2
-
 purified isozyme TAH II Hypocreales sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+/-)-epicatechin-gallate + H2O
-
 Hypocreales sp. epicatechin + gallate
-
 ?
(+/-)-epigallocatechin-3-gallate + H2O
-
 Hypocreales sp. epigallocatechin + gallate
-
 ?
m-digallate + H2O
-
 Hypocreales sp. gallate
-
 ?
additional information tannase cleaves ester bonds between gallic acid and glucose in tannic acid and m-digallic acid ester linkages, named depside bonds, it also hydrolyzes (+)-epicatechin gallate and (+)-epigallocatechin-3-gallate Hypocreales sp. ?
-
 ?
tannic acid + H2O
-
 Hypocreales sp. gallate + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE Hypocreales sp.

Synonyms

Synonyms Comment Organism
TAH
-
 Hypocreales sp.
tannin acyl hydrolase
-
 Hypocreales sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 isozyme TAH II Hypocreales sp.
25
-
 isozyme TAH I Hypocreales sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 temperature profile of the isozymes Hypocreales sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 temperature profile of the isozymes Hypocreales sp.
20 30 isozymes TAH I and TAH II, stable Hypocreales sp.
40 50 isozymes TAH I and TAH II, loss of 50-80% activity Hypocreales sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 isozymes TAH I and TAH II Hypocreales sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7.5 loss of 10-30% activity of TAH I and TAH II at "5°C and 4°C Hypocreales sp.