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Literature summary for 3.1.1.20 extracted from
- Purification and characterization of tannin acyl hydrolase from Aspergillus niger MTCC 2425 (2003), J. Basic Microbiol., 43, 449-461.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by EDTA | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 58% inhibition at 20 mM, noncompetitive | Aspergillus niger |  |
| Cd2+ | 55% inhibition at 20 mM, noncompetitive | Aspergillus niger | |
| Mg2+ | 33% inhibition at 20 mM, noncompetitive | Aspergillus niger | |
| Mn2+ | 54% inhibition at 20 mM, noncompetitive | Aspergillus niger | |
| additional information | not affected by EDTA | Aspergillus niger | |
| Zn2+ | 41% inhibition at 20 mM, noncompetitive | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
Tannic acid | - |
Aspergillus niger | |
| 0.95 | - |
methyl gallate | - |
Aspergillus niger | |
| 2.05 | - |
propyl gallate | - |
Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
x * 102000, SDS-PAGE, x * 83000, SDS-PAGE | Aspergillus niger |
| 102000 | - |
x * 102000, SDS-PAGE, x * 83000, SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger MTCC 2425 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| best at pH 5.5, overview, 51fold | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 355.6 | - |
purified enzyme | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl gallate + H2O | - |
Aspergillus niger | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Aspergillus niger MTCC 2425 | gallate + methanol | - |
? | |
| propyl gallate + H2O | - |
Aspergillus niger | gallate + propanol | - |
? | |
| propyl gallate + H2O | - |
Aspergillus niger MTCC 2425 | gallate + propanol | - |
? | |
| tannic acid + H2O | best substrate | Aspergillus niger | 10 gallate + D-glucose | - |
? | |
| tannic acid + H2O | best substrate | Aspergillus niger MTCC 2425 | 10 gallate + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 102000, SDS-PAGE, x * 83000, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tannin acyl hydrolase | - |
Aspergillus niger |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
Ca2+ | - |
Aspergillus niger | |
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