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Literature summary for 3.1.1.20 extracted from

  • Aguilar, C.N.; Gutierrez-Sanchez, G.
    Review: sources, properties, applications and potential uses of tannin acyl hydrolase (2001), Food Sci. Technol. Int., 7, 373-382.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information induction of enzyme by substrates Aspergillus niger
additional information induction of enzyme by substrates Rhizopus arrhizus
additional information induction of enzyme by substrates Aspergillus fischeri
additional information induction of enzyme by substrates Aspergillus flavus
additional information induction of enzyme by substrates Aspergillus oryzae
additional information induction of enzyme by substrates Trichoderma viride
additional information induction of enzyme by substrates Fusarium solani
additional information induction of enzyme by substrates Aspergillus japonicus

Application

Application Comment Organism
biotechnology production of the enzyme for industrial purposes Rhizopus arrhizus
biotechnology production of the enzyme for industrial purposes Aspergillus fischeri
biotechnology production of the enzyme for industrial purposes Aspergillus flavus
biotechnology production of the enzyme for industrial purposes Aspergillus oryzae
biotechnology production of the enzyme for industrial purposes Trichoderma viride
biotechnology production of the enzyme for industrial purposes Fusarium solani
biotechnology production of the enzyme for industrial purposes Aspergillus japonicus
biotechnology production of the enzyme for industrial purposes Cryphonectria parasitica
biotechnology production of the enzyme for industrial purposes, usage in quality improvement in the production of beer, wine Aspergillus niger

General Stability

General Stability Organism
a mixture of tannic acid and PEG 6000 protect the enzyme during purification Cryphonectria parasitica

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Aspergillus fischeri
2-mercaptoethanol
-
Aspergillus flavus
2-mercaptoethanol
-
Aspergillus japonicus
2-mercaptoethanol
-
Aspergillus niger
2-mercaptoethanol
-
Aspergillus oryzae
Ca2+
-
Aspergillus niger
Co2+
-
Aspergillus niger
Cu2+
-
Aspergillus fischeri
Cu2+
-
Aspergillus flavus
Cu2+
-
Aspergillus japonicus
Cu2+
-
Aspergillus niger
Cu2+
-
Aspergillus oryzae
EDTA
-
Aspergillus fischeri
EDTA
-
Aspergillus flavus
EDTA
-
Aspergillus japonicus
EDTA
-
Aspergillus niger
EDTA
-
Aspergillus oryzae
Fe2+
-
Aspergillus fischeri
Fe2+
-
Aspergillus flavus
Fe2+
-
Aspergillus japonicus
Fe2+
-
Aspergillus oryzae
Fe3+
-
Aspergillus niger
Mg2+
-
Aspergillus fischeri
Mg2+
-
Aspergillus flavus
Mg2+
-
Aspergillus japonicus
Mg2+
-
Aspergillus niger
Mg2+
-
Aspergillus oryzae
Mn2+
-
Aspergillus fischeri
Mn2+
-
Aspergillus flavus
Mn2+
-
Aspergillus japonicus
Mn2+
-
Aspergillus niger
Mn2+
-
Aspergillus oryzae
o-phenanthroline
-
Aspergillus fischeri
o-phenanthroline
-
Aspergillus flavus
o-phenanthroline
-
Aspergillus japonicus
o-phenanthroline
-
Aspergillus niger
o-phenanthroline
-
Aspergillus oryzae
PMSF
-
Aspergillus flavus
PMSF
-
Aspergillus niger
PMSF
-
Cryphonectria parasitica
Sodium thioglycolate
-
Aspergillus fischeri
Sodium thioglycolate
-
Aspergillus flavus
Sodium thioglycolate
-
Aspergillus japonicus
Sodium thioglycolate
-
Aspergillus niger
Sodium thioglycolate
-
Aspergillus oryzae
Zn2+
-
Aspergillus fischeri
Zn2+
-
Aspergillus flavus
Zn2+
-
Aspergillus japonicus
Zn2+
-
Aspergillus niger
Zn2+
-
Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Aspergillus oryzae 5618
-
extracellular
-
Aspergillus oryzae
-
-
extracellular
-
Aspergillus japonicus
-
-
membrane mucous membrane Bos taurus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
186000
-
-
Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
digallate + H2O Phaseolus vulgaris
-
gallate
-
?
digallate + H2O Bos taurus
-
gallate
-
?
digallate + H2O Aspergillus niger
-
gallate
-
?
digallate + H2O Rhizopus arrhizus
-
gallate
-
?
digallate + H2O Aspergillus fischeri
-
gallate
-
?
digallate + H2O Aspergillus flavus
-
gallate
-
?
digallate + H2O Aspergillus oryzae
-
gallate
-
?
digallate + H2O Trichoderma viride
-
gallate
-
?
digallate + H2O Fusarium solani
-
gallate
-
?
digallate + H2O Aspergillus japonicus
-
gallate
-
?
digallate + H2O Cryphonectria parasitica
-
gallate
-
?
methyl gallate + H2O Phaseolus vulgaris
-
gallic acid + methanol
-
?
methyl gallate + H2O Bos taurus
-
gallic acid + methanol
-
?
methyl gallate + H2O Aspergillus niger
-
gallic acid + methanol
-
?
methyl gallate + H2O Rhizopus arrhizus
-
gallic acid + methanol
-
?
methyl gallate + H2O Aspergillus fischeri
-
gallic acid + methanol
-
?
methyl gallate + H2O Aspergillus flavus
-
gallic acid + methanol
-
?
methyl gallate + H2O Aspergillus oryzae
-
gallic acid + methanol
-
?
methyl gallate + H2O Trichoderma viride
-
gallic acid + methanol
-
?
methyl gallate + H2O Fusarium solani
-
gallic acid + methanol
-
?
methyl gallate + H2O Aspergillus japonicus
-
gallic acid + methanol
-
?
methyl gallate + H2O Cryphonectria parasitica
-
gallic acid + methanol
-
?
additional information Phaseolus vulgaris production of phytate in beans ?
-
?
tannic acid + H2O Phaseolus vulgaris the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?
tannic acid + H2O Bos taurus the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?
tannic acid + H2O Aspergillus niger the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?
tannic acid + H2O Rhizopus arrhizus the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?
tannic acid + H2O Aspergillus fischeri the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?
tannic acid + H2O Aspergillus flavus the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?
tannic acid + H2O Aspergillus oryzae the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?
tannic acid + H2O Trichoderma viride the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?
tannic acid + H2O Fusarium solani the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?
tannic acid + H2O Aspergillus japonicus the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?
tannic acid + H2O Cryphonectria parasitica the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening 10 gallate + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus fischeri
-
-
-
Aspergillus flavus
-
-
-
Aspergillus japonicus
-
-
-
Aspergillus niger
-
-
-
Aspergillus oryzae
-
2 isozymes
-
Bos taurus
-
-
-
Cryphonectria parasitica
-
-
-
Fusarium solani
-
-
-
Phaseolus vulgaris
-
-
-
Rhizopus arrhizus
-
-
-
Trichoderma viride
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Phaseolus vulgaris
glycoprotein
-
Bos taurus
glycoprotein
-
Rhizopus arrhizus
glycoprotein
-
Aspergillus fischeri
glycoprotein
-
Aspergillus flavus
glycoprotein
-
Aspergillus oryzae
glycoprotein
-
Trichoderma viride
glycoprotein
-
Fusarium solani
glycoprotein
-
Aspergillus japonicus
glycoprotein
-
Cryphonectria parasitica
glycoprotein contains 43% sugar Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus
-
Aspergillus niger
-
Aspergillus fischeri
-
Aspergillus flavus
-
Aspergillus japonicus
142fold Cryphonectria parasitica
2 isoenzymes Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
intestine calf Bos taurus
-
additional information culture conditions Rhizopus arrhizus
-
additional information culture methods, overview Aspergillus niger
-
additional information culture methods, overview Aspergillus flavus
-
additional information culture methods, overview Aspergillus oryzae
-
additional information culture methods, overview Aspergillus japonicus
-
mycelium
-
Cryphonectria parasitica
-
rumen
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
170
-
purified enzyme Cryphonectria parasitica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
digallate + H2O
-
Phaseolus vulgaris gallate
-
?
digallate + H2O
-
Bos taurus gallate
-
?
digallate + H2O
-
Aspergillus niger gallate
-
?
digallate + H2O
-
Rhizopus arrhizus gallate
-
?
digallate + H2O
-
Aspergillus fischeri gallate
-
?
digallate + H2O
-
Aspergillus flavus gallate
-
?
digallate + H2O
-
Aspergillus oryzae gallate
-
?
digallate + H2O
-
Trichoderma viride gallate
-
?
digallate + H2O
-
Fusarium solani gallate
-
?
digallate + H2O
-
Aspergillus japonicus gallate
-
?
digallate + H2O
-
Cryphonectria parasitica gallate
-
?
methyl gallate + H2O
-
Phaseolus vulgaris gallic acid + methanol
-
?
methyl gallate + H2O
-
Bos taurus gallic acid + methanol
-
?
methyl gallate + H2O
-
Aspergillus niger gallic acid + methanol
-
?
methyl gallate + H2O
-
Rhizopus arrhizus gallic acid + methanol
-
?
methyl gallate + H2O
-
Aspergillus fischeri gallic acid + methanol
-
?
methyl gallate + H2O
-
Aspergillus flavus gallic acid + methanol
-
?
methyl gallate + H2O
-
Aspergillus oryzae gallic acid + methanol
-
?
methyl gallate + H2O
-
Trichoderma viride gallic acid + methanol
-
?
methyl gallate + H2O
-
Fusarium solani gallic acid + methanol
-
?
methyl gallate + H2O
-
Aspergillus japonicus gallic acid + methanol
-
?
methyl gallate + H2O
-
Cryphonectria parasitica gallic acid + methanol
-
?
additional information production of phytate in beans Phaseolus vulgaris ?
-
?
tannic acid + H2O
-
Phaseolus vulgaris 10 gallate + D-glucose
-
?
tannic acid + H2O
-
Bos taurus 10 gallate + D-glucose
-
?
tannic acid + H2O
-
Aspergillus niger 10 gallate + D-glucose
-
?
tannic acid + H2O
-
Rhizopus arrhizus 10 gallate + D-glucose
-
?
tannic acid + H2O
-
Aspergillus fischeri 10 gallate + D-glucose
-
?
tannic acid + H2O
-
Aspergillus flavus 10 gallate + D-glucose
-
?
tannic acid + H2O
-
Aspergillus oryzae 10 gallate + D-glucose
-
?
tannic acid + H2O
-
Trichoderma viride 10 gallate + D-glucose
-
?
tannic acid + H2O
-
Fusarium solani 10 gallate + D-glucose
-
?
tannic acid + H2O
-
Aspergillus japonicus 10 gallate + D-glucose
-
?
tannic acid + H2O
-
Cryphonectria parasitica 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Phaseolus vulgaris 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Bos taurus 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Aspergillus niger 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Rhizopus arrhizus 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Aspergillus fischeri 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Aspergillus flavus 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Aspergillus oryzae 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Trichoderma viride 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Fusarium solani 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Aspergillus japonicus 10 gallate + D-glucose
-
?
tannic acid + H2O the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening Cryphonectria parasitica 10 gallate + D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 80000-85000, strain N888 Aspergillus niger

Synonyms

Synonyms Comment Organism
TAH
-
Phaseolus vulgaris
TAH
-
Bos taurus
TAH
-
Aspergillus niger
TAH
-
Rhizopus arrhizus
TAH
-
Aspergillus fischeri
TAH
-
Aspergillus flavus
TAH
-
Aspergillus oryzae
TAH
-
Trichoderma viride
TAH
-
Fusarium solani
TAH
-
Aspergillus japonicus
TAH
-
Cryphonectria parasitica
tannin acyl hydrolase
-
Phaseolus vulgaris
tannin acyl hydrolase
-
Bos taurus
tannin acyl hydrolase
-
Aspergillus niger
tannin acyl hydrolase
-
Rhizopus arrhizus
tannin acyl hydrolase
-
Aspergillus fischeri
tannin acyl hydrolase
-
Aspergillus flavus
tannin acyl hydrolase
-
Aspergillus oryzae
tannin acyl hydrolase
-
Trichoderma viride
tannin acyl hydrolase
-
Fusarium solani
tannin acyl hydrolase
-
Aspergillus japonicus
tannin acyl hydrolase
-
Cryphonectria parasitica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
optimal growth and enzyme production temperature Aspergillus japonicus
30 40
-
Aspergillus fischeri
30 40
-
Aspergillus oryzae
35
-
-
Aspergillus niger
50 70
-
Aspergillus flavus
60
-
strain PKL-104 Aspergillus niger
70
-
strain N888 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 45
-
Aspergillus niger
4 45
-
Aspergillus flavus
30 60
-
Aspergillus fischeri
30 60
-
Aspergillus oryzae
30 60
-
Aspergillus japonicus
30 60
-
Cryphonectria parasitica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
esterase activity Aspergillus niger
5 5.5
-
Aspergillus flavus
5.5
-
strain PKL-104 Aspergillus niger
5.5 6
-
Aspergillus fischeri
5.5 6
-
Aspergillus oryzae
6
-
tannase activity, strain N888 Aspergillus niger
6.6
-
optimal growth and enzyme production pH value Aspergillus japonicus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus oryzae
-
-
3.5
Aspergillus niger strain N888
-
3.8
Aspergillus fischeri
-
-
4
Aspergillus flavus
-
-
4
Aspergillus japonicus
-
-
4
Aspergillus niger
-
4.4 4.3