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Literature summary for 3.1.1.2 extracted from

  • Kallnik, V.; Bunescu, A.; Sayer, C.; Bräsen, C.; Wohlgemuth, R.; Littlechild, J.; Siebers, B.
    Characterization of a phosphotriesterase-like lactonase from the hyperthermoacidophilic crenarchaeon Vulcanisaeta moutnovskia (2014), J. Biotechnol., 190, 11-17.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli Vulcanisaeta moutnovskia

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.05-10 mM, nearly complete loss of activity Vulcanisaeta moutnovskia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.19
-
4-nitrophenyl acetate pH 6.5, 50°C Vulcanisaeta moutnovskia

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ catalytic activity is strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) Vulcanisaeta moutnovskia
Co2+ catalytic activity is strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) Vulcanisaeta moutnovskia
Mn2+ catalytic activity is strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) Vulcanisaeta moutnovskia
Ni2+ catalytic activity is strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) Vulcanisaeta moutnovskia
Zn2+ catalytic activity is strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) Vulcanisaeta moutnovskia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, SDS-PAGE Vulcanisaeta moutnovskia
82000
-
gel filtration Vulcanisaeta moutnovskia

Organism

Organism UniProt Comment Textmining
Vulcanisaeta moutnovskia F0QXN6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Vulcanisaeta moutnovskia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Vulcanisaeta moutnovskia 4-nitrophenol + acetate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 36000, SDS-PAGE Vulcanisaeta moutnovskia

Synonyms

Synonyms Comment Organism
Vmut2255
-
Vulcanisaeta moutnovskia
VmutPLL
-
Vulcanisaeta moutnovskia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
substrate: methyl paraoxon Vulcanisaeta moutnovskia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 90 70°C: about 60% of maximal activity, 90°C: about 50% of maximal activity, substrate: methyl paraoxon Vulcanisaeta moutnovskia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
3 h, 30% loss of specific activity (substrate: methyl paraoxon) Vulcanisaeta moutnovskia
80
-
half-life: 2 h, substrate: methyl paraoxon Vulcanisaeta moutnovskia
90
-
half-life: 26 min, substrate: methyl paraoxon Vulcanisaeta moutnovskia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.66
-
4-nitrophenyl acetate pH 6.5, 50°C Vulcanisaeta moutnovskia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
substrate: methyl paraoxon Vulcanisaeta moutnovskia

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 30% of maximal activity, pH 9.0: about 80% of maximal activity, substrate: methyl paraoxon Vulcanisaeta moutnovskia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
4-nitrophenyl acetate pH 6.5, 50°C Vulcanisaeta moutnovskia