Literature summary for 3.1.1.2 extracted from

Valiyaveettil, M.; Alamneh, Y.; Biggemann, L.; Soojhawon, I.; Doctor, B.P.; Nambiar, M.P.
Efficient hydrolysis of the chemical warfare nerve agent tabun by recombinant and purified human and rabbit serum paraoxonase 1 (2010), Biochem. Biophys. Res. Commun., 403, 97-102.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Trichoplusia ni larvae	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
2-hydroxyquinoline	specific paraoxonase 1 inhibitor, dose-dependent inhibition	Homo sapiens
2-hydroxyquinoline	specific paraoxonase 1 inhibitor, dose-dependent inhibition	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sepharose column chromatography, Concanavalin A-Sepharose column chromatography, and Sepharose CL-6B gel filtration	Homo sapiens
DEAE-Sepharose column chromatography, Concanavalin A-Sepharose column chromatography, and Sepharose CL-6B gel filtration	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
serum	-	Homo sapiens	-
serum	-	Oryctolagus cuniculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.5	-	enzyme purified from serum, in 20 mM Tris-HCl buffer (pH 7.4), at 37°C	Homo sapiens
3.5	-	enzyme purified from serum, in 20 mM Tris-HCl buffer (pH 7.4), at 37°C	Oryctolagus cuniculus
8.5	-	recombinant enzyme, after purification, in 20 mM Tris-HCl buffer (pH 7.4), at 37°C	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Homo sapiens	4-nitrophenol + acetate	-	?
4-nitrophenyl acetate + H2O	-	Oryctolagus cuniculus	4-nitrophenol + acetate	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000-45000, paraoxonase 1 is separated as three bands with an approximate molecular weight of 40000-45000 Da, SDS-PAGE	Homo sapiens
?	x * 40000-45000, paraoxonase 1 is separated as three bands with an approximate molecular weight of 40000-45000 Da, SDS-PAGE	Oryctolagus cuniculus

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Release 2023.1 (January 2023)