Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.957 | - |
phenyl acetate | recombinant wild type enzyme, in 0.1 M Tris-HCl (pH 8.5), 2 M NaCl, and 2 mM CaCl2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27169 | - |
- |
Purification (Comment) | Organism |
---|---|
ceramic HA column chromatography, DEAE-Sepharose column chromatography, Sephadex G-25 gel filtration, and tert-butyl Sepharose column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.15 | - |
recombinant enzyme from crude cell extract | Homo sapiens |
465 | - |
recombinant enzyme after 90fold purification | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, purified recombinant enzyme in 20 mM Tris pH 7.7, 1 mM CaCl2, at least one month, remains stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenyl acetate + H2O | - |
Homo sapiens | phenol + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AREase | - |
Homo sapiens |