Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
ketoprofen ethyl ester | at pH 8.0 | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
estimated from amino acid sequence | Archaeoglobus fulgidus |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | organic solvents addition (acetone, acetonitrile, iso-propanol, ethanol, methanol, dimethyl sulfoxide, and dimethyl formamide) is not suitable for improvement of enzyme reaction through increase of substrate solubility | Archaeoglobus fulgidus |
Triton X-100 | 1060% relative activity in the presence of 1% (v/v) Triton X-100 | Archaeoglobus fulgidus |
Triton X-15 | 210% relative activity in the presence of 1% (v/v) Triton X-15 | Archaeoglobus fulgidus |
Triton X-165 | 1749% relative activity in the presence of 1% (v/v) Triton X-165 | Archaeoglobus fulgidus |
Triton X-305 | 1628% relative activity in the presence of 1% (v/v) Triton X-305 | Archaeoglobus fulgidus |
Triton X-45 | 662% relative activity in the presence of 1% (v/v) Triton X-45 | Archaeoglobus fulgidus |
Tween 20 | 1396% relative activity in the presence of 1% (v/v) Tween 20 | Archaeoglobus fulgidus |
Tween 40 | 1306% relative activity in the presence of 1% (v/v) Tween 40 | Archaeoglobus fulgidus |
Tween 60 | 1396% relative activity in the presence of 1% (v/v) Tween 60 | Archaeoglobus fulgidus |
Tween 80 | 1339% relative activity in the presence of 1% (v/v) Tween 80 | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
strain DSM 4304 | - |
Purification (Comment) | Organism |
---|---|
Ni+2-NTA resin chromatography | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | highest specific activity | Archaeoglobus fulgidus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprylate + H2O | 10% activity compared to 4-nitrophenyl butyrate | Archaeoglobus fulgidus | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + hexanoate | - |
? | |
ketoprofen ethyl ester + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
additional information | no activity with 4-nitrophenyl laurate and 4-nitrophenyl palmitate | Archaeoglobus fulgidus | ? | - |
? | |
additional information | the enzyme is a carboxylesterase and has highest activity with 4-nitrophenyl butyrate, activity decreases with acylglycerols containing longer carbon chains | Archaeoglobus fulgidus | ? | - |
? | |
phenyl acetate + H2O | - |
Archaeoglobus fulgidus | phenol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27500, estimated from amino acid sequence | Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
using ketoprofen ethyl ester as substrate | Archaeoglobus fulgidus |
80 | - |
using 4-nitrophenyl butyrate as substrate | Archaeoglobus fulgidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | 10% of activity is retained after 3 h at 90°C using ketoprofen ethyl ester as substrate, the enzyme shows about 50% activity at 40°C, 68% relative activity at 50°C, about 90% activity at 60°C, 100% activity at 70°C, about 90% activity at 80°C, and 58% activity at 90°C | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
no loss of activity after 180 min at 65°C | Archaeoglobus fulgidus |
70 | - |
no loss of activity after 180 min at 70°C | Archaeoglobus fulgidus |
75 | - |
about 20% loss of activity after 180 min at 75°C | Archaeoglobus fulgidus |
80 | - |
about 30% loss of activity after 180 min at 80°C | Archaeoglobus fulgidus |
85 | - |
about 40% loss of activity after 180 min at 85°C | Archaeoglobus fulgidus |
90 | - |
about 50% loss of activity after 80 min at 90°C | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Archaeoglobus fulgidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 12 | enzyme activity is decreased higher than pH 8.0, no activity at pH 3.0 and 12.0, about 10% relative activity is retained at pH 4 and 11 | Archaeoglobus fulgidus |