Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate | the enzyme is activated by 87% by incubation with 1% (w/v) 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate, at 30°C | Saccharolobus solfataricus | |
dimethyl sulfoxide | 21% increase of activity at 50% (v/v) dimethyl sulfoxide, at 30°C | Saccharolobus solfataricus | |
ethanol | 15% increase of activity at 50% (v/v) ethanol, at 30°C | Saccharolobus solfataricus | |
Lubrol | the enzyme is activated by 14% by incubation with 1% Lubrol, at 30°C | Saccharolobus solfataricus | |
paraoxon | 115 increase of activita in the presence of 0.5 mM paraoxon, after 30 min at 30°C | Saccharolobus solfataricus | |
pyridoxal 5'-phosphate | 110% activity in the presence of 5 mM pyridoxal 5'-phosphate, after 30 min at 30°C | Saccharolobus solfataricus | |
Tween 20 | the enzyme is activated by 67% by incubation for 60 min with 1% (v/v) Tween 20 at 30°C | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
C105S | mutant shows 24.0% activity compared to the wild type enzyme | Saccharolobus solfataricus |
C107S | mutant shows 30.8% activity compared to the wild type enzyme | Saccharolobus solfataricus |
C129S | mutant shows 3.6% activity compared to the wild type enzyme | Saccharolobus solfataricus |
D251N | mutant shows 0.6% activity compared to the wild type enzyme | Saccharolobus solfataricus |
H281N | mutant shows 19.4% activity compared to the wild type enzyme | Saccharolobus solfataricus |
S156A | mutant shows 0.012% activity compared to the wild type enzyme | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-propanol | 55% residual activity in the presence of 90% (v/v) 2-propanol, after 60 min at 70°C | Saccharolobus solfataricus | |
acetonitrile | 43% residual activity in the presence of 90% (v/v) acetonitrile, after 60 min at 70°C | Saccharolobus solfataricus | |
diethyl dicarbonate | 6% residual activity in the presence of 5 mM diethyl dicarbonate, after 30 min at 30°C | Saccharolobus solfataricus | |
diisopropylfluorophosphate | complete inactivation at 5 mM diisopropylfluorophosphate, after 30 min at 30°C | Saccharolobus solfataricus | |
dimethyl sulfoxide | 71% residual activity in the presence of 90% (v/v) dimethyl sulfoxide, after 60 min at 70°C | Saccharolobus solfataricus | |
EDTA | 92% residual activity in the presence of 10 mM EDTA, after 30 min at 75°C | Saccharolobus solfataricus | |
eserine | 92% residual activity in the presence of 5 mM eserine, after 30 min at 30°C | Saccharolobus solfataricus | |
ethanol | 63% residual activity in the presence of 90% (v/v) ethanol, after 60 min at 70°C | Saccharolobus solfataricus | |
Hg2+ | complete inactivation at 5 mM Hg2+, after 30 min at 30°C | Saccharolobus solfataricus | |
methanol | 48% residual activity in the presence of 90% (v/v) methanol, after 60 min at 70°C | Saccharolobus solfataricus | |
NaCl | 76% residual activity in the presence of 2 M NaCl, after 60 min at 70°C | Saccharolobus solfataricus | |
p-chloromercuribenzoate | 23% residual activity in the presence of 5 mM p-chloromercuribenzoate, after 30 min at 30°C | Saccharolobus solfataricus | |
Phenylglyoxal | 97% residual activity in the presence of 5 mM phenylglyoxal, after 30 min at 30°C | Saccharolobus solfataricus | |
phenylmethylsulfonyl fluoride | 23% residual activity in the presence of 5 mM phenylmethylsulfonyl fluoride, after 30 min at 30°C | Saccharolobus solfataricus | |
SDS | about half of the activity is retained at 1% SDS after incubation for 60 min at 70°C, the addition of 5% SDS completely eliminates the enzyme activity at 70°C | Saccharolobus solfataricus | |
Triton X-100 | 11% residual activity in the presence of 5% (v/v) Tween 80, after 60 min at 70°C | Saccharolobus solfataricus | |
Tween 80 | 21% residual activity in the presence of 5% (v/v) Tween 80, after 60 min at 70°C | Saccharolobus solfataricus | |
Urea | 88% residual activity in the presence of 8 M urea, after 60 min at 70°C | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
phenyl acetate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
0.028 | - |
4-nitrophenyl caprylate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
0.029 | - |
Alpha-naphthyl acetate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
0.031 | - |
4-nitrophenyl caproate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
0.081 | - |
4-nitrophenyl caprate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
0.122 | - |
4-nitrophenyl laurate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
0.224 | - |
4-nitrophenyl butyrate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
0.234 | - |
4-nitrophenyl phosphate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
0.276 | - |
4-nitrophenyl palmitate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35175 | - |
1 * 35175, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
35180 | - |
MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
37000 | - |
SDS-PAGE | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
Triton X-100 | after incubation for 60 min with Triton X-100 the enzyme activity is remarkably decreased with increasing temperature and concentration | Saccharolobus solfataricus |
urea | the purified enzyme shows high stability against urea | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | B5BLW5 | - |
- |
Saccharolobus solfataricus P1 | B5BLW5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme is purified by DEAE-cellulose column chromatography, butyl-Sepharose column chromatography, Q-Sepharose column chromatography, and hydroxyapatite column chromatography | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant enzyme from crude cell extract, using phenyl acetate as substrate, at 60°C and pH 7.0 | Saccharolobus solfataricus |
5580 | - |
recombinant enzyme after 858.5fold purification, using phenyl acetate as substrate, at 60°C and pH 7.0 | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Saccharolobus solfataricus P1 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Saccharolobus solfataricus P1 | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl diethylphosphate + H2O | - |
Saccharolobus solfataricus | diethylphosphate + 4-nitrophenol | - |
? | |
4-nitrophenyl laurate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Saccharolobus solfataricus P1 | 4-nitrophenol + phosphate | - |
? | |
alpha-naphthyl acetate + H2O | - |
Saccharolobus solfataricus | alpha-naphthol + acetate | - |
? | |
alpha-naphthyl acetate + H2O | - |
Saccharolobus solfataricus P1 | alpha-naphthol + acetate | - |
? | |
phenyl acetate + H2O | - |
Saccharolobus solfataricus | phenol + acetate | - |
? | |
phenyl acetate + H2O | - |
Saccharolobus solfataricus P1 | phenol + acetate | - |
? | |
tributyrin + H2O | - |
Saccharolobus solfataricus | dibutyrin + butyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35175, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
arylesterase | the enzyme is a serine esterase belonging to the A-esterases and contains a catalytic triad composed of Ser156, Asp251, and His281 in the active site | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
94 | - |
- |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 94 | the enzyme retains 52% of its activity after 50 h of incubation at 90°C, the enzyme is rapidly inactivated above 94°C, most of the enzyme activity is maintained for 5 days at 50°C, the enzyme activity gradually decreases with time at higher temperatures, approximately 70% of the enzyme activity remains after 5 days at 70°C, and 52% of it is still preserved after 50 h at 90°C, however, at 90°C after 5 days, the enzyme is completely inactivated | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
264 | - |
4-nitrophenyl laurate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
265 | - |
4-nitrophenyl palmitate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
269 | - |
4-nitrophenyl phosphate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
285 | - |
4-nitrophenyl butyrate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
341 | - |
4-nitrophenyl caprate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
410 | - |
4-nitrophenyl caprylate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
450 | - |
Alpha-naphthyl acetate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
467 | - |
4-nitrophenyl caproate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus | |
521 | - |
phenyl acetate | recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Saccharolobus solfataricus |