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Literature summary for 3.1.1.2 extracted from

  • Kanamori-Kataoka, M.; Seto, Y.
    Paraoxonase activity against nerve gases measured by capillary electrophoresis and characterization of human serum paraoxonase (PON1) polymorphism in the coding region (Q192R) (2009), Anal. Biochem., 385, 94-100.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Q192R the mutant shows lower Vmax and kcat/Km values for soman and sarin than the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.86
-
phenyl acetate wild type enzyme, in 50 mM Tris-HCl with 1 mM CaCl2 (pH 8.0), at 25°C Homo sapiens
0.89
-
phenyl acetate mutant enzyme Q192R, in 50 mM Tris-HCl with 1 mM CaCl2 (pH 8.0), at 25°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27169
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
blue agarose affinity chromatography and HiTrap DEAE column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
4°C, purified PON1 in DEAE buffer, at least two weeks, remains stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-naphthyl acetate + H2O
-
Homo sapiens 2-naphthol + acetate
-
?
phenyl acetate + H2O
-
Homo sapiens phenol + acetate
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Homo sapiens