Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens | |
Mg2+ | Mg2, Mn2+ and other divalent metal ions can not substitute for Ca2+ and lead to a loss od arylesterase activity | Homo sapiens | |
Mn2+ | Mg2, Mn2+ and other divalent metal ions can not substitute for Ca2+ and lead to a loss od arylesterase activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
a high level of recombinant hPON1 type A (rPON1A) is produced by Hi-5 insect cells. A fraction is secreted into the cell culture medium, but the majority remains associated with the host insect cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, completely and irreversibly inhibits activity with phenyl acetate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
phenyl acetate | pH 8.0, wild-type enzyme | Homo sapiens | |
3.8 | - |
phenyl acetate | pH 8.0, recombinant enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains high-mannose N-glycan chains. Enzymatic deglycosylation does not inhibit antioxidant activity of rPON1A | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, the purified rPON1A maintains stability for several months when stored in the presence of 0.1% Tergitol NP-10 and 1 mM CaCl2 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenyl acetate + H2O | - |
Homo sapiens | phenol + acetate | - |
? | |
phenylacetate + H2O | - |
Homo sapiens | phenol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | Homo sapiens |