Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.2 extracted from

  • Brushia, R.J.; Forte, T.M.; Oda, M.N.; La Du, B.N.; Bielicki, J.K.
    Baculovirus-mediated expression and purification of human serum paraoxonase 1A (2001), J. Lipid Res., 42, 951-958.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ required Homo sapiens
Mg2+ Mg2, Mn2+ and other divalent metal ions can not substitute for Ca2+ and lead to a loss od arylesterase activity Homo sapiens
Mn2+ Mg2, Mn2+ and other divalent metal ions can not substitute for Ca2+ and lead to a loss od arylesterase activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
a high level of recombinant hPON1 type A (rPON1A) is produced by Hi-5 insect cells. A fraction is secreted into the cell culture medium, but the majority remains associated with the host insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, completely and irreversibly inhibits activity with phenyl acetate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
phenyl acetate pH 8.0, wild-type enzyme Homo sapiens
3.8
-
phenyl acetate pH 8.0, recombinant enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains high-mannose N-glycan chains. Enzymatic deglycosylation does not inhibit antioxidant activity of rPON1A Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Storage Stability

Storage Stability Organism
4°C, the purified rPON1A maintains stability for several months when stored in the presence of 0.1% Tergitol NP-10 and 1 mM CaCl2 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenyl acetate + H2O
-
Homo sapiens phenol + acetate
-
?
phenylacetate + H2O
-
Homo sapiens phenol + acetate
-
?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Homo sapiens