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Literature summary for 3.1.1.2 extracted from

  • Fenster, K.M.; Parkin, K.L.; Steele, J.L.
    Characterization of an arylesterase from Lactobacillus helveticus CNRZ32 (2000), J. Appl. Microbiol., 88, 572-583.
    View publication on PubMed
Search for more literature for 3.1.1.2

Activating Compound

Activating Compound Comment Organism Structure
NaCl optimal concentration is 3.5% at 35°C Lactobacillus helveticus

Cloned(Commentary)

Cloned (Comment) Organism
gene estA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha as C-terminally HIs-tagged protein Lactobacillus helveticus

Protein Variants

Protein Variants Comment Organism
C105A site-directed mutagenesis, 55.9% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
H137A site-directed mutagenesis, 17.4% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
H164A site-directed mutagenesis, inactive mutant Lactobacillus helveticus
H56A site-directed mutagenesis, 30.0% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
S10A site-directed mutagenesis, inactive mutant Lactobacillus helveticus
S112A site-directed mutagenesis, 99.5% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
S138G site-directed mutagenesis, 99.2% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
S155A site-directed mutagenesis, 99.0% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
S42A site-directed mutagenesis, 33.2% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus
S44A site-directed mutagenesis, 33.7% of the wild-type activity with substrate 4-nitrophenyl butyrate Lactobacillus helveticus

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
 Lactobacillus helveticus
additional information no inhibition by EDTA and iodoacetic acid Lactobacillus helveticus
pepstatin A
-
 Lactobacillus helveticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22600 25100 gel filtration on Sephacryl 100 and 300 Lactobacillus helveticus
23400
-
 1 * 23400, SDS-PAGE Lactobacillus helveticus

Organism

Organism UniProt Comment Textmining
Lactobacillus helveticus Q9LAH7 enzyme EstA, gene estA
-
Lactobacillus helveticus CNRZ32 Q9LAH7 enzyme EstA, gene estA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli, to homogeneity Lactobacillus helveticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Lactobacillus helveticus
486 519 purified recombinant wild-type enzyme, substrate phenyl acetate Lactobacillus helveticus
623
-
 purified recombinant wild-type enzyme, substrate phenyl acetate in presence of 3.5% NaCl Lactobacillus helveticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-acetoxypyridine + H2O
-
 Lactobacillus helveticus ?
-
 ?
4-acetoxybenzaldehyde + H2O
-
 Lactobacillus helveticus ?
-
 ?
4-nitrophenyl acetate + H2O
-
 Lactobacillus helveticus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Lactobacillus helveticus CNRZ32 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Lactobacillus helveticus 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caproate + H2O
-
 Lactobacillus helveticus 4-nitrophenol + caproate
-
 ?
4-nitrophenyl caprylate + H2O
-
 Lactobacillus helveticus 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl propionate + H2O
-
 Lactobacillus helveticus 4-nitrophenol + propionate
-
 ?
4-nitrophenyl propionate + H2O
-
 Lactobacillus helveticus CNRZ32 4-nitrophenol + propionate
-
 ?
4-nitrophenyl valerate + H2O
-
 Lactobacillus helveticus 4-nitrophenol + valerate
-
 ?
additional information substrate specificity, no activity with 4-nitrophenyl caprate, 4-nitrophenyl laurate, 4-nitrophenyl phosphate, 4-nitrophenyl sulfate, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl phenylphosphonate, stearoyl-CoA, palmitoyl-CoA, methylacetylsalicylate, ethyl acetate, propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, isoamyl acetate, and caproyl acetate Lactobacillus helveticus ?
-
 ?
additional information substrate specificity, no activity with 4-nitrophenyl caprate, 4-nitrophenyl laurate, 4-nitrophenyl phosphate, 4-nitrophenyl sulfate, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl phenylphosphonate, stearoyl-CoA, palmitoyl-CoA, methylacetylsalicylate, ethyl acetate, propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, isoamyl acetate, and caproyl acetate Lactobacillus helveticus CNRZ32 ?
-
 ?
phenyl acetate + H2O best substrate Lactobacillus helveticus phenol + acetate
-
 ?
phenyl acetate + H2O best substrate Lactobacillus helveticus CNRZ32 phenol + acetate
-
 ?
phenylthioacetate + H2O
-
 Lactobacillus helveticus thiophenol + acetate
-
 ?
phenylthioacetate + H2O
-
 Lactobacillus helveticus CNRZ32 thiophenol + acetate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 23400, SDS-PAGE Lactobacillus helveticus

Synonyms

Synonyms Comment Organism
ESt-A
-
 Lactobacillus helveticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Lactobacillus helveticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Lactobacillus helveticus

pI Value

Organism Comment pI Value Maximum pI Value
Lactobacillus helveticus isoelectric focusing
-
 8