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Literature summary for 3.1.1.17 extracted from
- Structural basis of the gamma-lactone-ring formation in ascorbic acid biosynthesis by the senescence marker protein-30/gluconolactonase (2013), PLoS ONE, 8, e53706.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mus musculus |
| expressed in Escherichia coli BL21(DE3)-Rossetta2/pLysS cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 1.6 M ammonium sulfate and 100 mM Tris-HCl, pH 8.5 | Mus musculus |
| hanging-dop vapor diffusion method, using 28% (w/v) PEG6000, 100 mM Tris-HCl pH 7.5, and 5 mM CaCl2 | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Homo sapiens |
| 34000 | - |
x * 34000, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucono-1,5-lactone + H2O | Homo sapiens | - |
D-gluconate | - |
? |  |
| D-glucono-1,5-lactone + H2O | Mus musculus | - |
D-gluconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q15493 | - |
- |
| Mus musculus | Q64374 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Toyopearl DEAE-650M column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Homo sapiens |
| Toyopearl DEAE-650M column chromatography, Toyopearl SuperQ-650M column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucono-1,5-lactone + H2O | - |
Homo sapiens | D-gluconate | - |
? | |
| D-glucono-1,5-lactone + H2O | - |
Mus musculus | D-gluconate | - |
? | |
| xylitol + H2O | - |
Homo sapiens | ? | - |
? | |
| xylitol + H2O | - |
Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Homo sapiens |
| ? | x * 34000, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GNL | - |
Homo sapiens |
| GNL | - |
Mus musculus |
| regucalcin | - |
Homo sapiens |
| regucalcin | - |
Mus musculus |
| senescence marker protein-30 | - |
Homo sapiens |
| senescence marker protein-30 | - |
Mus musculus |
| SMP30 | - |
Homo sapiens |
| SMP30 | - |
Mus musculus |
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Release 2023.1 (January 2023)
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