Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hydrogen peroxide | stimulates activity at 0.1-0.5 mM | Euglena gracilis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Euglena gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | strong inhibition | Euglena gracilis | |
additional information | not inhibited by L-ascorbic acid and dithiothreitol | Euglena gracilis | |
N-ethylmaleimide | - |
Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.55 | - |
L-galactonic acid | recombinant enzyme | Euglena gracilis | |
1.67 | - |
L-galactono-1,4-lactone | recombinant enzyme | Euglena gracilis | |
2.08 | - |
D-gulono-1,5-lactone | recombinant enzyme | Euglena gracilis | |
3.05 | - |
L-gulono-1,4-lactone | recombinant enzyme | Euglena gracilis | |
4.55 | - |
L-Gulonic acid | recombinant enzyme | Euglena gracilis | |
9.4 | - |
D-glucono-1,5-lactone | wild type enzyme | Euglena gracilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mn2+, Co2+, Mg2+, and Ca2+ have a significant low effect on the activity to 19, 9, 8, and 7%, respectively | Euglena gracilis | |
Zn2+ | the enzyme requires a divalent metal ion for maximum activity which is obtained by the addition of 0.075 mM Zn2+ | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
about 34000 Da, recombinant enzyme, SDS-PAGE | Euglena gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | A9CPS8 | - |
- |
Euglena gracilis Z | A9CPS8 | - |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
wild type enzyme, using L-galactono-1,4-lactone as substrate | Euglena gracilis |
2.7 | - |
wild type enzyme, using L-gulono-1,4-lactone as substrate | Euglena gracilis |
5.4 | - |
wild type enzyme, using L-glucono-1,5-lactone as substrate | Euglena gracilis |
9.4 | - |
wild type enzyme, using D-galactono-1,4-lactone as substrate | Euglena gracilis |
12 | - |
wild type enzyme, using D-gulono-1,4-lactone as substrate | Euglena gracilis |
20.1 | - |
recombinant enzyme, using D-gulono-1,4-lactone as substrate | Euglena gracilis |
24.5 | - |
recombinant enzyme, using L-galactono-1,4-lactone as substrate | Euglena gracilis |
27.3 | - |
recombinant enzyme, using D-galactono-1,4-lactone as substrate | Euglena gracilis |
39 | - |
recombinant enzyme, using D-glucono-1,5-lactone as substrate | Euglena gracilis |
40.9 | - |
recombinant enzyme, using L-gulono-1,4-lactone as substrate | Euglena gracilis |
217 | - |
wild type enzyme, using D-glucono-1,5-lactone as substrate | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactonic acid + H2O | - |
Euglena gracilis | ? | - |
? | |
D-galactonic acid + H2O | - |
Euglena gracilis Z | ? | - |
? | |
D-galactono-1,4-lactone + H2O | wild type enzyme shows 4.33% specific activity, recombinant enzyme shows 70% specific activity compared to the reaction with D-glucono-1,5-lactone | Euglena gracilis | ? | - |
r | |
D-galactono-1,4-lactone + H2O | wild type enzyme shows 4.33% specific activity, recombinant enzyme shows 70% specific activity compared to the reaction with D-glucono-1,5-lactone | Euglena gracilis Z | ? | - |
r | |
D-glucono-1,5-lactone + H2O | wild type and recombinant enzyme show 100% specific activity | Euglena gracilis | D-gluconate | - |
r | |
D-glucono-1,5-lactone + H2O | wild type and recombinant enzyme show 100% specific activity | Euglena gracilis Z | D-gluconate | - |
r | |
D-gulono-1,4-lactone + H2O | wild type enzyme shows 12.0% specific activity, recombinant enzyme shows 20.1% specific activity compared to the reaction with D-glucono-1,5-lactone | Euglena gracilis | D-galactonate | - |
r | |
D-gulono-1,4-lactone + H2O | wild type enzyme shows 12.0% specific activity, recombinant enzyme shows 20.1% specific activity compared to the reaction with D-glucono-1,5-lactone | Euglena gracilis Z | D-galactonate | - |
r | |
D-gulono-1,5-lactone + H2O | - |
Euglena gracilis | ? | - |
? | |
L-galactonic acid + H2O | - |
Euglena gracilis | ? | - |
? | |
L-galactono-1,4-lactone + H2O | wild type enzyme shows 0.37% specific activity, recombinant enzyme shows 62.8% specific activity compared to the reaction with D-glucono-1,5-lactone | Euglena gracilis | ? | - |
r | |
L-glucono-1,5-lactone + H2O | wild type enzyme shows 2.49% specific activity, recombinant enzyme shows no activity compared to the reaction with D-glucono-1,5-lactone | Euglena gracilis | L-gluconate | - |
r | |
L-glucono-1,5-lactone + H2O | wild type enzyme shows 2.49% specific activity, recombinant enzyme shows no activity compared to the reaction with D-glucono-1,5-lactone | Euglena gracilis Z | L-gluconate | - |
r | |
L-gulonic acid + H2O | - |
Euglena gracilis | ? | - |
? | |
L-gulono-1,4-lactone + H2O | wild type enzyme shows 1.24% specific activity, recombinant enzyme shows 105% specific activity compared to the reaction with D-glucono-1,5-lactone | Euglena gracilis | L-gulonate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ALase | - |
Euglena gracilis |
aldonolactonase | - |
Euglena gracilis |
SMP30 | - |
Euglena gracilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
D-gulono-1,5-lactone | recombinant enzyme | Euglena gracilis | |
192 | - |
D-glucono-1,5-lactone | wild type enzyme | Euglena gracilis | |
450 | - |
L-galactono-1,4-lactone | recombinant enzyme | Euglena gracilis | |
615 | - |
L-galactonic acid | recombinant enzyme | Euglena gracilis | |
1040 | - |
L-gulono-1,4-lactone | recombinant enzyme | Euglena gracilis | |
1503 | - |
L-Gulonic acid | recombinant enzyme | Euglena gracilis |