Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.14 extracted from

  • Khalyfa, A.; Kermasha, S.; Marsot, P.; Goetghebeur, M.
    Purification and characterization of chlorophyllase from alga Phaeodactylum tricornutum by preparative native electrophoresis (1995), Appl. Biochem. Biotechnol., 53, 11-27.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol in combination with Mg2+ dithiothreitol can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum
Lipids plant membrane lipids, including phosphatidylserine, phosphatidylglycerol, and beta-carotene increase activity Phaeodactylum tricornutum

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
Phaeodactylum tricornutum
phytol
-
Phaeodactylum tricornutum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ in combination with dithiothreitol Mg2+ can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000 + x * 46000, SDS-PAGE Phaeodactylum tricornutum
46000
-
x * 43000 + x * 46000, SDS-PAGE Phaeodactylum tricornutum

Organism

Organism UniProt Comment Textmining
Phaeodactylum tricornutum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Phaeodactylum tricornutum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Phaeodactylum tricornutum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll a + H2O
-
Phaeodactylum tricornutum chlorophyllide a + phytol
-
?
chlorophyll b + H2O
-
Phaeodactylum tricornutum chlorophyllide b + phytol
-
?

Subunits

Subunits Comment Organism
? x * 43000 + x * 46000, SDS-PAGE Phaeodactylum tricornutum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
31
-
-
Phaeodactylum tricornutum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Phaeodactylum tricornutum