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Literature summary for 3.1.1.14 extracted from

  • Chen, L.O.; Lin, C.; Kelkar, S.M.; Chang, Y.; Shaw, J.
    Transgenic broccoli (Brassica oleracea var. italica) with antisense chlorophyllase (BoCLH1) delays postharvest yellowing (2008), Plant Sci., 174, 25-31.
    View publication on PubMed
Search for more literature for 3.1.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression of antisense construct of gene CLH3 in broccoli plants using Agrobacterium tumefaciens-mediated transformation Brassica oleracea
expression of antisense constructs of gene CLH1 in broccoli plants using Agrobacterium tumefaciens-mediated transformation Brassica oleracea
expression of antisense constructs of gene CLH2 in broccoli plants using Agrobacterium tumefaciens-mediated transformation Brassica oleracea

Protein Variants

Protein Variants Comment Organism
additional information a chimeric construct with the antisense gene BoCLH1, driven by the CaMV 35S promoter and Nos-terminator and harboring the hygromycin resistance gene, is used for Agrobacterium tumefaciens-mediated transformation, effects of the antisense BoCLH1 gene on the postharvest senescence of broccoli, phenotype with slower postharvest yellowing during storage in the dark at 20°C, overview Brassica oleracea
additional information no effects on yellowing by antisense construct of gene CHL2 Brassica oleracea
additional information no effects on yellowing by antisense constructs of gene CHL3 Brassica oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyll + H2O Brassica oleracea chlorophyllase is the first enzyme in the degradation pathway of chlorophyll, resulting in postharvest yellowing in broccoli phytol + chlorophyllide
-
 ?
chlorophyll + H2O Brassica oleracea chlorophyllase is the first enzyme in the degradation pathway of chlorophyll, resulting in postharvest yellowing in broccoli, CLH1 is expressed during the course of broccoli postharvest senescence phytol + chlorophyllide
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica oleracea Q8GTM2 CLH1; var. italica, gene CLH3
-
Brassica oleracea Q8GTM3 CLH2; var. italica, gene CLH2
-
Brassica oleracea Q8GTM4 CLH1; var. italica, gene CLH1
-

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
 Brassica oleracea
-
leaf
-
 Brassica oleracea
-
additional information CLH1 is expressed during the course of broccoli postharvest senescence Brassica oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll + H2O
-
 Brassica oleracea phytol + chlorophyllide
-
 ?
chlorophyll + H2O chlorophyllase is the first enzyme in the degradation pathway of chlorophyll, resulting in postharvest yellowing in broccoli Brassica oleracea phytol + chlorophyllide
-
 ?
chlorophyll + H2O chlorophyllase is the first enzyme in the degradation pathway of chlorophyll, resulting in postharvest yellowing in broccoli, CLH1 is expressed during the course of broccoli postharvest senescence Brassica oleracea phytol + chlorophyllide
-
 ?

Synonyms

Synonyms Comment Organism
CLH1
-
 Brassica oleracea
CLH2
-
 Brassica oleracea
CLH3
-
 Brassica oleracea