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Literature summary for 3.1.1.14 extracted from

  • Harpaz-Saad, S.; Azoulay, T.; Arazi, T.; Ben-Yaakov, E.; Mett, A.; Shiboleth, Y.M.; Hoertensteiner, S.; Gidoni, D.; Gal-On, A.; Goldschmidt, E.E.; Eyal, Y.
    Chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated (2007), Plant Cell, 19, 1007-1022.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
Chlase gene, DNA and amino acid sequence determination and anaylsis, expression of precursor full-length wild-type Chlase and of a mutant ChlaseDELTAN lacking the N-terminal 21 amino acids, which corresponds to the mature enzyme, in two heterologous plant systems: in Cucurbita pepo cv. Maย’ayan plants using a ZYMV-based viral vector infective clone system and inoculation of cotyledons, and transiently in Nicotiana tabacum cv. Samsun NN chloroplast membranes of protoplasts, expression of full-length and truncated enzyme versions as thioredoxin fusion proteins in Escherichia coli Citrus sinensis

Protein Variants

Protein Variants Comment Organism
additional information expression of full-length citrus Chlase results in limited chlorophyll breakdown in tobacco protoplasts and no visible leaf phenotype in whole plants, whereas expression of a Chlase version lacking the N-terminal 21 amino acids, i.e. ChlaseDELTAN, which corresponds to the mature protein, leads to extensive chlorophyll breakdown in both tobacco protoplasts and squash leaves, mutant ChlaseDELTAN-expressing squash leaves display a dramatic chlorotic phenotype in plants grown under low-intensity light, whereas under natural light a lesion-mimic phenotype occurrs, which follows the accumulation of chlorophyllide, a photodynamic chlorophyll breakdown product, phenotypes, overview Citrus sinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast membrane
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Citrus sinensis 31969
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyll + H2O Citrus sinensis Chlase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated, it catalyzes the cleavage of the hydrophobic thylakoid-anchoring phytol chain of chlorophyll from the porphyrin ring, resulting in the product chlorophyllide, which retains the typical green color, chlorophyll catabolic pathway, overview phytol + chlorophyllide
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Organism

Organism UniProt Comment Textmining
Citrus sinensis Q9MV14 gene clh
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Purification (Commentary)

Purification (Comment) Organism
recombinant enzymes in the membrane fraction from Nicotiana tabacum chloroplasts by ultracentrifugation Citrus sinensis

Source Tissue

Source Tissue Comment Organism Textmining
leaf
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Citrus sinensis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll + H2O Chlase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated, it catalyzes the cleavage of the hydrophobic thylakoid-anchoring phytol chain of chlorophyll from the porphyrin ring, resulting in the product chlorophyllide, which retains the typical green color, chlorophyll catabolic pathway, overview Citrus sinensis phytol + chlorophyllide
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chlorophyll a + H2O chlorophyll a substrate from Petroselinum sativum leaves Citrus sinensis phytol + chlorophyllide
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Synonyms

Synonyms Comment Organism
Chlase
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Citrus sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
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assay at Citrus sinensis