Cloned (Comment) | Organism |
---|---|
Chlase gene, DNA and amino acid sequence determination and anaylsis, expression of precursor full-length wild-type Chlase and of a mutant ChlaseDELTAN lacking the N-terminal 21 amino acids, which corresponds to the mature enzyme, in two heterologous plant systems: in Cucurbita pepo cv. Maยayan plants using a ZYMV-based viral vector infective clone system and inoculation of cotyledons, and transiently in Nicotiana tabacum cv. Samsun NN chloroplast membranes of protoplasts, expression of full-length and truncated enzyme versions as thioredoxin fusion proteins in Escherichia coli | Citrus sinensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of full-length citrus Chlase results in limited chlorophyll breakdown in tobacco protoplasts and no visible leaf phenotype in whole plants, whereas expression of a Chlase version lacking the N-terminal 21 amino acids, i.e. ChlaseDELTAN, which corresponds to the mature protein, leads to extensive chlorophyll breakdown in both tobacco protoplasts and squash leaves, mutant ChlaseDELTAN-expressing squash leaves display a dramatic chlorotic phenotype in plants grown under low-intensity light, whereas under natural light a lesion-mimic phenotype occurrs, which follows the accumulation of chlorophyllide, a photodynamic chlorophyll breakdown product, phenotypes, overview | Citrus sinensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast membrane | - |
Citrus sinensis | 31969 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | Citrus sinensis | Chlase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated, it catalyzes the cleavage of the hydrophobic thylakoid-anchoring phytol chain of chlorophyll from the porphyrin ring, resulting in the product chlorophyllide, which retains the typical green color, chlorophyll catabolic pathway, overview | phytol + chlorophyllide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus sinensis | Q9MV14 | gene clh | - |
Purification (Comment) | Organism |
---|---|
recombinant enzymes in the membrane fraction from Nicotiana tabacum chloroplasts by ultracentrifugation | Citrus sinensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Citrus sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | Chlase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated, it catalyzes the cleavage of the hydrophobic thylakoid-anchoring phytol chain of chlorophyll from the porphyrin ring, resulting in the product chlorophyllide, which retains the typical green color, chlorophyll catabolic pathway, overview | Citrus sinensis | phytol + chlorophyllide | - |
? | |
chlorophyll a + H2O | chlorophyll a substrate from Petroselinum sativum leaves | Citrus sinensis | phytol + chlorophyllide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Chlase | - |
Citrus sinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Citrus sinensis |