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Literature summary for 3.1.1.14 extracted from

  • Tsuchiya, T.; Suzuki, T.; Yamada, T.; Shimada, H.; Masuda, T.; Ohta, H.; Takamiya, K.
    Chlorophyllase as a serine hydrolase: identification of a putative catalytic triad (2003), Plant Cell Physiol., 44, 96-101.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Chenopodium album

Protein Variants

Protein Variants Comment Organism
C234A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Chenopodium album
C248A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Chenopodium album
C282A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Chenopodium album
D191N site-directed mutagenesis, inactive mutant Chenopodium album
D264N site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Chenopodium album
H100A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Chenopodium album
H161A site-directed mutagenesis, inactive mutant Chenopodium album
H241A site-directed mutagenesis, about 90% reduced activity compared to the wild-type enzyme Chenopodium album
H254A site-directed mutagenesis, nearly inactive mutant Chenopodium album
H254Y site-directed mutagenesis, about 90% reduced activity compared to the wild-type enzyme Chenopodium album
H262A site-directed mutagenesis, nearly inactive mutant Chenopodium album
H262Y site-directed mutagenesis, nearly inactive mutant Chenopodium album
H81A site-directed mutagenesis, about 85% reduced activity compared to the wild-type enzyme Chenopodium album
S162A site-directed mutagenesis, nearly inactive mutant Chenopodium album
S167A site-directed mutagenesis, about 50% reduced activity compared to the wild-type enzyme Chenopodium album

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate irreversible, 91% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C Chenopodium album
iodoacetamide irreversible, 6% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C Chenopodium album
N-ethylmaleimide irreversible, 15.9% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C Chenopodium album
PCMB irreversible, 92.6% inhibition at 1 mM, 1 h preincubation at pH 7.0 and 30°C Chenopodium album
PMSF irreversible, 89.2% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C Chenopodium album

Organism

Organism UniProt Comment Textmining
Chenopodium album
-
-
-

Reaction

Reaction Comment Organism Reaction ID
chlorophyll + H2O = phytol + chlorophyllide also catalyzes chlorophyllide transfer, e.g. converts chlorophyll to methylchlorophyllide, catalytic mechanism, catalytic triad is composed of Ser162, Asp191, and His262, and reveals the enzyme as a serine hydrolase Chenopodium album

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll + H2O
-
Chenopodium album phytol + chlorophyllide
-
?

Synonyms

Synonyms Comment Organism
Chlase
-
Chenopodium album
CLH
-
Chenopodium album