Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Chenopodium album |
Protein Variants | Comment | Organism |
---|---|---|
C234A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Chenopodium album |
C248A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Chenopodium album |
C282A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Chenopodium album |
D191N | site-directed mutagenesis, inactive mutant | Chenopodium album |
D264N | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Chenopodium album |
H100A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Chenopodium album |
H161A | site-directed mutagenesis, inactive mutant | Chenopodium album |
H241A | site-directed mutagenesis, about 90% reduced activity compared to the wild-type enzyme | Chenopodium album |
H254A | site-directed mutagenesis, nearly inactive mutant | Chenopodium album |
H254Y | site-directed mutagenesis, about 90% reduced activity compared to the wild-type enzyme | Chenopodium album |
H262A | site-directed mutagenesis, nearly inactive mutant | Chenopodium album |
H262Y | site-directed mutagenesis, nearly inactive mutant | Chenopodium album |
H81A | site-directed mutagenesis, about 85% reduced activity compared to the wild-type enzyme | Chenopodium album |
S162A | site-directed mutagenesis, nearly inactive mutant | Chenopodium album |
S167A | site-directed mutagenesis, about 50% reduced activity compared to the wild-type enzyme | Chenopodium album |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl fluorophosphate | irreversible, 91% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C | Chenopodium album | |
iodoacetamide | irreversible, 6% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C | Chenopodium album | |
N-ethylmaleimide | irreversible, 15.9% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C | Chenopodium album | |
PCMB | irreversible, 92.6% inhibition at 1 mM, 1 h preincubation at pH 7.0 and 30°C | Chenopodium album | |
PMSF | irreversible, 89.2% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C | Chenopodium album |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chenopodium album | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
chlorophyll + H2O = phytol + chlorophyllide | also catalyzes chlorophyllide transfer, e.g. converts chlorophyll to methylchlorophyllide, catalytic mechanism, catalytic triad is composed of Ser162, Asp191, and His262, and reveals the enzyme as a serine hydrolase | Chenopodium album |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | - |
Chenopodium album | phytol + chlorophyllide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Chlase | - |
Chenopodium album |
CLH | - |
Chenopodium album |