Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 13% stimulation at 1 mM, 24 h preincubation | Capsicum annuum | |
dithiothreitol | 13% stimulation at 1 mM, 24 h preincubation | Capsicum annuum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | 93% inhibition at 1 mM, 24 h preincubation | Capsicum annuum | |
Co2+ | 56% inhibition at 0.01 mM, 24 h preincubation | Capsicum annuum | |
Cu2+ | 55% inhibition at 0.01 mM, 24 h preincubation | Capsicum annuum | |
Fe2+ | 89% inhibition at 0.01 mM, 24 h preincubation | Capsicum annuum | |
Fe3+ | 13% inhibition at 0.01 mM, 24 h preincubation | Capsicum annuum | |
Hg2+ | 85% inhibition at 0.01 mM, 24 h preincubation | Capsicum annuum | |
Mg2+ | 12% inhibition at 0.01 mM, 24 h preincubation | Capsicum annuum | |
Mn2+ | 67% inhibition at 0.01 mM, 24 h preincubation | Capsicum annuum | |
additional information | poor inhibition by methyl methanethiosulfonate and iodoacetamide | Capsicum annuum | |
N-ethylmaleimide | 13% inhibition at 1 mM, 24 h preincubation | Capsicum annuum | |
pheophytin b | substrate inhibition above 0.005 mM | Capsicum annuum | |
Zn2+ | 60% inhibition at 0.01 mM, 24 h preincubation | Capsicum annuum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
pheophytin a | pH 7.5, 35°C | Capsicum annuum | |
0.004 | - |
chlorophyll b | pH 7.5, 35°C | Capsicum annuum | |
0.0064 | - |
pheophytin b | pH 7.5, 35°C | Capsicum annuum | |
0.011 | - |
chlorophyll a | pH 7.5, 35°C | Capsicum annuum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Capsicum annuum | 9507 | - |
chromoplast | - |
Capsicum annuum | 9509 | - |
thylakoid membrane | intrinsic | Capsicum annuum | 42651 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll a/b + H2O | Capsicum annuum | - |
phytol + chlorophyllide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum annuum | - |
cv. Agridulce | - |
Purification (Comment) | Organism |
---|---|
partially | Capsicum annuum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
chlorophyll + H2O = phytol + chlorophyllide | also catalyzes chlorophyllide transfer, e.g. converts chlorophyll to methylchlorophyllide | Capsicum annuum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | green, fresh or ripening | Capsicum annuum | - |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 1 year without loss of activity | Capsicum annuum |
25°C, loss of 50% activity within 6 days | Capsicum annuum |
4°C, loss of 50% within 18 days | Capsicum annuum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll a + H2O | best substrate, stereospecific for the substrate, no activity with the C13-epimer | Capsicum annuum | phytol + chlorophyllide | - |
? | |
chlorophyll a/b + H2O | - |
Capsicum annuum | phytol + chlorophyllide | - |
? | |
chlorophyll b + H2O | stereospecific for the substrate, no activity with the C13-epimer | Capsicum annuum | phytol + chlorophyllide | - |
? | |
pheophytin a + H2O | - |
Capsicum annuum | phytol + pheophorbide | - |
? | |
pheophytin b + H2O | - |
Capsicum annuum | phytol + pheophorbide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Chlase | - |
Capsicum annuum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Capsicum annuum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | - |
Capsicum annuum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable for 1 h | Capsicum annuum |
50 | - |
degradation after 40 min, more rapid at higher temperatures | Capsicum annuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Capsicum annuum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11.5 | 35% of maximal activity at pH 7.0, 60& of maximal activity at pH 11.5 | Capsicum annuum |