Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.13 extracted from

  • Imanaka, T.; Amanuma-Muto, K.; Ohkuma, S.; Takano, T.
    Characterization of lysosomal acid lipase purified from rabbit liver (1984), J. Biochem., 96, 1089-1101.
    View publication on PubMed
Search for more literature for 3.1.1.13

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition at 2 mM Oryctolagus cuniculus
diethyldicarbonate complete inactivation at 2 mM Oryctolagus cuniculus
diisopropylfluorophosphate 30% inhibition at 0.2 mM Oryctolagus cuniculus
p-bromophenacylbromide strong inhibition at 0.2 mM Oryctolagus cuniculus
p-chloromercuriphenyl sulfonic acid 0.2 mM strongly inhibitory Oryctolagus cuniculus
Phenylmethylsulfonylfluoride 30% inhibition at 0.2 mM Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 4-methylumbelliferyl oleate
-
 Oryctolagus cuniculus
0.04
-
 cholesteryl oleate
-
 Oryctolagus cuniculus
0.05
-
 triolein
-
 Oryctolagus cuniculus
0.07
-
 diolein
-
 Oryctolagus cuniculus
0.2
-
 monoolein
-
 Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Oryctolagus cuniculus 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Oryctolagus cuniculus
42000
-
 SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acylglycerols + H2O Oryctolagus cuniculus
-
 ? + fatty acids
-
 ?
cholesteryl esters + H2O Oryctolagus cuniculus
-
 cholesterol + fatty acid
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
 rabbit
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
25000fold to homogeneity, chromatography steps, preparative slab gel electrophoresis, affinity chromatography Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Storage Stability

Storage Stability Organism
-70°C, 40% ethylene glycol, 5 mM 2-mercaptoethanol, 1 month Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl oleate + H2O
-
 Oryctolagus cuniculus 4-methylumbelliferone + oleic acid
-
 ?
acylglycerols + H2O
-
 Oryctolagus cuniculus ? + fatty acids
-
 ?
cholesteryl esters + H2O
-
 Oryctolagus cuniculus cholesterol + fatty acid
-
 ?
cholesteryl oleate + H2O
-
 Oryctolagus cuniculus cholesterol + oleic acid
-
 ?
diolein + H2O
-
 Oryctolagus cuniculus monooleoylglycerol + oleic acid
-
 ?
monoolein + H2O
-
 Oryctolagus cuniculus glycerol + oleic acid
-
 ?
triolein + H2O
-
 Oryctolagus cuniculus dioleoylglycerol + oleic acid
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000-42000 SDS-PAGE, gel filtration Oryctolagus cuniculus

Synonyms

Synonyms Comment Organism
acid lipase
-
 Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 50% inactivation Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3
-
 for cholesteryl oleate Oryctolagus cuniculus
4.5
-
 hydrolysis of tri-di- and monoolein Oryctolagus cuniculus
5
-
 for 4-methyl-umbelliferyl oleate Oryctolagus cuniculus