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Literature summary for 3.1.1.13 extracted from

  • Calero-Rueda, O.; Barba, V.; Rodriguez, E.; Plou, F.; Martinez, A.T.; Martinez, M.J.
    Study of a sterol esterase secreted by Ophiostoma piceae: sequence, model and biochemical properties (2009), Biochim. Biophys. Acta, 1794, 1099-1106.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris, resulting in higher activities than those obtained in the Ophistoma piceae cultures Ophiostoma piceae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58200
-
MALDI-TOF, SDS-PAGE Ophiostoma piceae

Organism

Organism UniProt Comment Textmining
Ophiostoma piceae Q2TFW1
-
-

Purification (Commentary)

Purification (Comment) Organism
The Ophiostoma piceae esterase is purified from 15-day-old cultures on glucose-peptone-yeast extract medium supplemented with 0.5% olive oil. Recombinant protein is purified following the same procedure from 7-day-old cultures growing on BMMY medium Ophiostoma piceae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
79.84
-
substrate cholesteryl oleate, native enzyme in the presence of 5% Genapol X-100 Ophiostoma piceae
171
-
substrate triolein, native enzyme in the presence of 5% Genapol X-100 Ophiostoma piceae
319
-
substrate cholesteryl oleate, recombinant enzyme in the presence of 5% Genapol X-100 Ophiostoma piceae
449.7
-
substrate 4-nitrophenyl butyrate, native enzyme in the presence of 1% Genapol X-100 Ophiostoma piceae
698.1
-
substrate triolein, recombinant enzyme in the presence of 5% Genapol X-100 Ophiostoma piceae
1336
-
substrate 4-nitrophenyl butyrate, recombinant enzyme in the presence of 1% Genapol X-100 Ophiostoma piceae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl oleate + H2O
-
Ophiostoma piceae cholesterol + oleate
-
?
p-nitrophenyl butyrate + H2O
-
Ophiostoma piceae p-nitrophenol + butanoic acid
-
?
p-nitrophenyl palmitate + H2O
-
Ophiostoma piceae p-nitrophenol + palmitic acid
-
?
tributyrin + H2O
-
Ophiostoma piceae dibutyrin + butyrate
-
?
triolein + H2O
-
Ophiostoma piceae diolein + oleate
-
?

Synonyms

Synonyms Comment Organism
OPE
-
Ophiostoma piceae
Sterol esterase
-
Ophiostoma piceae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Ophiostoma piceae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Ophiostoma piceae