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Literature summary for 3.1.1.13 extracted from

  • Kontkanen, H.; Tenkanen, M.; Fagerstrom, R.; Reinikainen, T.
    Characterisation of steryl esterase activities in commercial lipase preparations (2004), J. Biotechnol., 108, 51-59.
    View publication on PubMed
Search for more literature for 3.1.1.13

Organism

Organism UniProt Comment Textmining
Chromobacterium viscosum
-
 enzyme activity in commercial lipase preparation from Asahi, comparison of different preparations
-
Diutina rugosa
-
 enzyme activity in commercial lipase preparation from Amano, comparison of different preparations
-
Pseudomonas sp.
-
 enzyme activity in commercial lipase preparation from Amano, comparison of different preparations
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.024
-
 substrate cholesteryl stearate, pH 7.0 Pseudomonas sp.
0.12
-
 substrate cholesteryl oleate, pH 7.0 Pseudomonas sp.
0.18
-
 substrate cholesteryl linoleate, pH 7.0 Pseudomonas sp.
0.18
-
 substrate cholesteryl linolenate, pH 7.0 Pseudomonas sp.
1.2
-
 substrate cholesteryl stearate, pH 7.0 Diutina rugosa
1.98
-
 substrate cholesteryl stearate, pH 7.0 Chromobacterium viscosum
3.1
-
 substrate cholesteryl palmitate, pH 7.0 Pseudomonas sp.
4.6
-
 substrate cholesteryl linoleate, pH 7.0 Chromobacterium viscosum
4.6
-
 substrate cholesteryl linolenate, pH 7.0 Chromobacterium viscosum
4.8
-
 substrate cholesteryl oleate, pH 7.0 Chromobacterium viscosum
5
-
 substrate cholesteryl palmitate, pH 7.0 Diutina rugosa
6.9
-
 substrate cholesteryl linoleate, pH 7.0 Diutina rugosa
7
-
 substrate cholesteryl oleate, pH 7.0 Diutina rugosa
7.8
-
 substrate cholesteryl palmitate, pH 7.0 Chromobacterium viscosum
11
-
 substrate cholesteryl linolenate, pH 7.0 Diutina rugosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl linoleate + H2O
-
 Pseudomonas sp. cholesterol + linoleic acid
-
 ?
cholesteryl linoleate + H2O
-
 Diutina rugosa cholesterol + linoleic acid
-
 ?
cholesteryl linoleate + H2O
-
 Chromobacterium viscosum cholesterol + linoleic acid
-
 ?
cholesteryl linolenate + H2O
-
 Pseudomonas sp. cholesterol + linolenic acid
-
 ?
cholesteryl linolenate + H2O
-
 Diutina rugosa cholesterol + linolenic acid
-
 ?
cholesteryl linolenate + H2O
-
 Chromobacterium viscosum cholesterol + linolenic acid
-
 ?
cholesteryl oleate + H2O
-
 Pseudomonas sp. cholesterol + oleic acid
-
 ?
cholesteryl oleate + H2O
-
 Diutina rugosa cholesterol + oleic acid
-
 ?
cholesteryl oleate + H2O
-
 Chromobacterium viscosum cholesterol + oleic acid
-
 ?
cholesteryl palmitate + H2O
-
 Pseudomonas sp. cholesterol + palmitic acid
-
 ?
cholesteryl palmitate + H2O
-
 Diutina rugosa cholesterol + palmitic acid
-
 ?
cholesteryl palmitate + H2O
-
 Chromobacterium viscosum cholesterol + palmitic acid
-
 ?
cholesteryl stearate + H2O
-
 Pseudomonas sp. cholesterol + stearic acid
-
 ?
cholesteryl stearate + H2O
-
 Diutina rugosa cholesterol + stearic acid
-
 ?
cholesteryl stearate + H2O
-
 Chromobacterium viscosum cholesterol + stearic acid
-
 ?
additional information in aqueous dispersion, reaction proceeds slowly. Addition of surfactant Thesit accelerates initial reaction rate Diutina rugosa ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Diutina rugosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 over 80% of maximum activity within this range Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 3 h, 80% residual activity Pseudomonas sp.
50
-
 3 h, 80% residual activity Diutina rugosa