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Literature summary for 3.1.1.13 extracted from

  • Xiang, H.; Takaya, N.; Hoshino, T.
    Novel cholesterol esterase secreted by Streptomyces persists during aqueous long-term storage (2006), J. Biosci. Bioeng., 101, 19-25.
    View publication on PubMed
Search for more literature for 3.1.1.13

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 0.3%, about 50% activation Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 94% residual activity Streptomyces sp.
Ag+ 1 mM, 75% residual activity Streptomyces sp.
deoxycholic acid 1 mM, 60% inhibition Streptomyces sp.
dithiothreitol 1 mM, 0% residual activity Streptomyces sp.
Hg2+ 1 mM, 50% residual activity Streptomyces sp.
additional information not inhibitory: phenylmethylsulfonylfluoride Streptomyces sp.
p-chloromercuribenzoate 1 mM, 78% residual activity Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 cholesteryl linoleate pH 7.0, 37°C Streptomyces sp.
0.34
-
 cholesteryl myristate pH 7.0, 37°C Streptomyces sp.
1.1
-
 p-nitrophenyl myristate pH 7.0, 37°C Streptomyces sp.
2.1
-
 p-nitrophenyl palmitate pH 7.0, 37°C Streptomyces sp.
4.3
-
 p-nitrophenyl laurate pH 7.0, 37°C Streptomyces sp.
6.8
-
 cholesteryl palmitate pH 7.0, 37°C Streptomyces sp.
7
-
 cholesteryl stearate pH 7.0, 37°C Streptomyces sp.
7
-
 cholesteryl acetate pH 7.0, 37°C Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23600
-
 x * 23600, SDS-PAGE Streptomyces sp.
163000
-
 gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. X9
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 substrate p-nitrophenyl acetate, pH 7.0, 37°C Streptomyces sp.
0.67
-
 substrate p-nitrophenyl laurate, pH 7.0, 37°C Streptomyces sp.
2.08
-
 substrate p-nitrophenyl palmitate, pH 7.0, 37°C Streptomyces sp.
4.97
-
 substrate p-nitrophenyl myristate, pH 7.0, 37°C Streptomyces sp.
11.8
-
 substrate cholesteryl acetate, pH 7.0, 37°C Streptomyces sp.
13.4
-
 substrate cholesteryl palmitate, pH 7.0, 37°C Streptomyces sp.
13.4
-
 substrate cholesteryl stearate, pH 7.0, 37°C Streptomyces sp.
41
-
 substrate cholesteryl myristate, pH 7.0, 37°C Streptomyces sp.
78.8
-
 substrate cholesteryl linoleate, pH 7.0, 37°C Streptomyces sp.

Storage Stability

Storage Stability Organism
stable during long-term aqueous storage at room temperature Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl acetate + H2O
-
 Streptomyces sp. cholesterol + acetic acid
-
 ?
cholesteryl linoleate + H2O
-
 Streptomyces sp. cholesterol + linoleic acid
-
 ?
cholesteryl linoleate + H2O
-
 Streptomyces sp. X9 cholesterol + linoleic acid
-
 ?
cholesteryl myristate + H2O
-
 Streptomyces sp. cholesterol + myristic acid
-
 ?
cholesteryl myristate + H2O
-
 Streptomyces sp. X9 cholesterol + myristic acid
-
 ?
cholesteryl palmitate + H2O
-
 Streptomyces sp. cholesterol + palmitic acid
-
 ?
cholesteryl palmitate + H2O
-
 Streptomyces sp. X9 cholesterol + palmitic acid
-
 ?
cholesteryl stearate + H2O
-
 Streptomyces sp. cholesterol + stearic acid
-
 ?
cholesteryl stearate + H2O
-
 Streptomyces sp. X9 cholesterol + stearic acid
-
 ?
p-nitrophenyl acetate + H2O
-
 Streptomyces sp. p-nitrophenol + acetate
-
 ?
p-nitrophenyl acetate + H2O
-
 Streptomyces sp. X9 p-nitrophenol + acetate
-
 ?
p-nitrophenyl laurate + H2O
-
 Streptomyces sp. p-nitrophenol + laurate
-
 ?
p-nitrophenyl myristate + H2O
-
 Streptomyces sp. p-nitrophenol + myristic acid
-
 ?
p-nitrophenyl palmitate + H2O
-
 Streptomyces sp. p-nitrophenol + palmitic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 23600, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 30 min, 60% residual activity Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 p-nitrophenyl laurate pH 7.0, 37°C Streptomyces sp.
2.9
-
 p-nitrophenyl palmitate pH 7.0, 37°C Streptomyces sp.
4.5
-
 p-nitrophenyl myristate pH 7.0, 37°C Streptomyces sp.
23.5
-
 cholesteryl myristate pH 7.0, 37°C Streptomyces sp.
31.2
-
 cholesteryl linoleate pH 7.0, 37°C Streptomyces sp.
70.8
-
 cholesteryl stearate pH 7.0, 37°C Streptomyces sp.
96.7
-
 cholesteryl palmitate pH 7.0, 37°C Streptomyces sp.
110
-
 cholesteryl acetate pH 7.0, 37°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7.5
-
 Streptomyces sp.