Literature summary for 3.1.1.13 extracted from

Milad-Kodsi, E.; Langston, T.B.; Gergis, M.R.; Grogan, W.M.
Acidic residues emulate a phosphorylation switch to enhance the activity of rat hepatic neutral cytosolic cholesterol esterase (2005), Biochim. Biophys. Acta, 1734, 62-73.

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Protein Variants

Protein Variants	Comment	Organism
G505D/S506D	18fold increase in activity against cholesterol oleate, decrease in activity against p-nitrophenyl octanoate	Rattus norvegicus
G505N/S506N/K496Q/R503Q	no activity with cholesterol oleate, reduced activity with p-nitrophenyl substrates	Rattus norvegicus
G505N/S506N/R503Q	no activity	Rattus norvegicus
K496Q/R503Q	no activity with cholesterol oleate, or p-nitrophenyl octanoate, reduced activity with p-nitrophenyl acetate	Rattus norvegicus
additional information	mutation S506D plus insertion of D in position 507, no activity with cholesterol oleate, reduced activity with p-nitrophenyl substrates	Rattus norvegicus
S506D	50% decrease in activity against cholesterol oleate, increase in activity against p-nitrophenyl octanoate, nitrophenyl acetate	Rattus norvegicus
S506K	no activity with cholesterol oleate, somehow reduced activity with p-nitrophenyl substrates	Rattus norvegicus
S506N	no activity with cholesterol oleate, somehow reduced activity with p-nitrophenyl substrates	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.144	-	p-nitrophenyl octanoate	mutant G505D/S506D, pH 7.4, 37°C	Rattus norvegicus
0.316	-	p-nitrophenyl octanoate	mutant S506D, pH 7.4, 37°C	Rattus norvegicus
1.639	-	p-nitrophenyl octanoate	mutant S506N, pH 7.4, 37°C	Rattus norvegicus
1.782	-	p-nitrophenyl octanoate	mutant S506K, pH 7.4, 37°C	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	different activity against p-nitrophenyl and cholesteryl substrates is influenced by phosphorylation state of S506	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cholesteryl oleate + H2O	-	Rattus norvegicus	cholesterol + oleic acid	-	?
nitrophenyl acetate + H2O	-	Rattus norvegicus	p-nitrophenol + acetate	-	?
p-nitrophenyl octanoate + H2O	-	Rattus norvegicus	p-nitrophenol + octanoic acid	-	?

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Release 2023.1 (January 2023)