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Literature summary for 3.1.1.13 extracted from
- Determination of total cholesterol in serum by cholesterol esterase and cholesterol oxidase immobilized and co-immobilized on to arylamine glass (2002), Biotechnol. Appl. Biochem., 35, 191-197.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | immobilization of the enzyme alone or coimmobilization with cholesterol oxidase on zirkonia-coated acrylamine glass beads for analysis of total cholesterol in serum, long-term stability, precise measurements with the enzyme mixture at cholesterol concentration above 1 g/l | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Bos taurus | - |
| pancreas | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in distilled water, 3 months during regular use in analysis, 30% loss of activity for the free enzyme, 17% loss of activity for the immobilized enzyme, 18% loss of activity for the coimmobilized enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl acetate + H2O | - |
Bos taurus | cholesterol + acetate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cholesterol esterase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
coimmobilized enzyme | Bos taurus |
| 37 | - |
assay at | Bos taurus |
| 40 | - |
optimum, immobilized and free enzyme | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
30 min, 18% remaining activity for the free enzyme, 43% remaining activity for the immobilized enzyme, 48% remaining activity for the coimmobilized enzyme | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
free enzyme, coimmobilized enzyme | Bos taurus |
| 7.5 | - |
immobilized enzyme | Bos taurus |
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Release 2023.1 (January 2023)
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