Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no interfacial activation | Ophiostoma piceae | |
olive oil | strongly induces enzyme production in liquid cultures, 0.5% v/v | Ophiostoma piceae |
Application | Comment | Organism |
---|---|---|
paper production | enzyme has biotechnological application in paper manufacturing since it efficiently hydrolyzes both triglycerides and sterol esters, which form pitch deposits during manufacturing of softwood and hardwood paper pulps, respectively | Ophiostoma piceae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 20% inhibition at 10 mM | Ophiostoma piceae | |
Fe3+ | 56% inhibition at 10 mM | Ophiostoma piceae | |
Hg2+ | 54% inhibition at 10 mM | Ophiostoma piceae | |
additional information | no inhibition by Na+, Ag+, NH4+, Mg2+, Ca2+, Zn2+, Pb2+, NaN3, KCN, 2-mercaptoethanol, and PMSF | Ophiostoma piceae | |
SDS | 33% inhibition at 1% | Ophiostoma piceae | |
Triton X-100 | 39% inhibition at 0.1% | Ophiostoma piceae | |
Tween 20 | 15% inhibition at 1% | Ophiostoma piceae | |
Tween 40 | 40% inhibition at 1% | Ophiostoma piceae | |
Tween 60 | 48% inhibition at 1% | Ophiostoma piceae | |
Tween 80 | 51% inhibition at 1% | Ophiostoma piceae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
4-nitrophenyl butyrate | pH 7.0, 37°C | Ophiostoma piceae | |
0.33 | - |
4-nitrophenyl palmitate | pH 7.0, 37°C | Ophiostoma piceae | |
0.87 | - |
cholesteryl palmitate | pH 7.0, 37°C | Ophiostoma piceae | |
0.98 | - |
triolein | pH 7.0, 37°C | Ophiostoma piceae | |
0.99 | - |
cholesteryl linoleate | pH 7.0, 37°C | Ophiostoma piceae | |
1 | - |
cholesteryl oleate | pH 7.0, 37°C | Ophiostoma piceae | |
1.1 | - |
cholesteryl stearate | pH 7.0, 37°C | Ophiostoma piceae | |
3 | - |
cholesteryl butyrate | pH 7.0, 37°C | Ophiostoma piceae | |
9.9 | - |
tributyrin | pH 7.0, 37°C | Ophiostoma piceae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Ophiostoma piceae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56500 | - |
x * 56500, SDS-PAGE | Ophiostoma piceae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ophiostoma piceae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme contains 8% N-linked carbohydrate | Ophiostoma piceae |
Purification (Comment) | Organism |
---|---|
2.4fold | Ophiostoma piceae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Ophiostoma piceae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
121 | - |
purified enzyme | Ophiostoma piceae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Ophiostoma piceae | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Ophiostoma piceae | 4-nitrophenol + palmitate | - |
? | |
cholesteryl butyrate + H2O | - |
Ophiostoma piceae | cholesterol + butyrate | - |
? | |
cholesteryl linoleate + H2O | - |
Ophiostoma piceae | cholesterol + linoleate | - |
? | |
cholesteryl oleate + H2O | - |
Ophiostoma piceae | cholesterol + oleate | - |
? | |
cholesteryl palmitate + H2O | - |
Ophiostoma piceae | cholesterol + palmitate | - |
? | |
cholesteryl stearate + H2O | - |
Ophiostoma piceae | cholesterol + stearate | - |
? | |
additional information | no activity with triacetin, enzyme activity increases with increasing chain lebgth and number of double bonds, no interfacial activation | Ophiostoma piceae | ? | - |
? | |
tributyrin + H2O | - |
Ophiostoma piceae | dibutyrin + butyrate | - |
? | |
triolein + H2O | best substrate | Ophiostoma piceae | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56500, SDS-PAGE | Ophiostoma piceae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ophiostoma piceae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
24 h, loss of 20% activity | Ophiostoma piceae |
45 | - |
24 h, loss of 75% activity | Ophiostoma piceae |
60 | - |
inactivation | Ophiostoma piceae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44 | - |
4-nitrophenyl butyrate | pH 7.0, 37°C | Ophiostoma piceae | |
47 | - |
cholesteryl butyrate | pH 7.0, 37°C | Ophiostoma piceae | |
67 | - |
cholesteryl palmitate | pH 7.0, 37°C | Ophiostoma piceae | |
71 | - |
cholesteryl stearate | pH 7.0, 37°C | Ophiostoma piceae | |
74 | - |
4-nitrophenyl palmitate | pH 7.0, 37°C | Ophiostoma piceae | |
138 | - |
cholesteryl oleate | pH 7.0, 37°C | Ophiostoma piceae | |
150 | - |
cholesteryl linoleate | pH 7.0, 37°C | Ophiostoma piceae | |
179 | - |
tributyrin | pH 7.0, 37°C | Ophiostoma piceae | |
290 | - |
triolein | pH 7.0, 37°C | Ophiostoma piceae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | substrate 4-nitrophenyl butyrate | Ophiostoma piceae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ophiostoma piceae | isoelectric focusing | - |
3.3 |