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Literature summary for 3.1.1.13 extracted from

  • Calero-Rueda, O.; Plou, F.J.; Ballesteros, A.; Martinez, A.T.; Martinez, M.J.
    Production, isolation and characterization of a sterol esterase from Ophiostoma piceae (2002), Biochim. Biophys. Acta, 1599, 28-35.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no interfacial activation Ophiostoma piceae
olive oil strongly induces enzyme production in liquid cultures, 0.5% v/v Ophiostoma piceae

Application

Application Comment Organism
paper production enzyme has biotechnological application in paper manufacturing since it efficiently hydrolyzes both triglycerides and sterol esters, which form pitch deposits during manufacturing of softwood and hardwood paper pulps, respectively Ophiostoma piceae

Inhibitors

Inhibitors Comment Organism Structure
EDTA 20% inhibition at 10 mM Ophiostoma piceae
Fe3+ 56% inhibition at 10 mM Ophiostoma piceae
Hg2+ 54% inhibition at 10 mM Ophiostoma piceae
additional information no inhibition by Na+, Ag+, NH4+, Mg2+, Ca2+, Zn2+, Pb2+, NaN3, KCN, 2-mercaptoethanol, and PMSF Ophiostoma piceae
SDS 33% inhibition at 1% Ophiostoma piceae
Triton X-100 39% inhibition at 0.1% Ophiostoma piceae
Tween 20 15% inhibition at 1% Ophiostoma piceae
Tween 40 40% inhibition at 1% Ophiostoma piceae
Tween 60 48% inhibition at 1% Ophiostoma piceae
Tween 80 51% inhibition at 1% Ophiostoma piceae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
4-nitrophenyl butyrate pH 7.0, 37°C Ophiostoma piceae
0.33
-
4-nitrophenyl palmitate pH 7.0, 37°C Ophiostoma piceae
0.87
-
cholesteryl palmitate pH 7.0, 37°C Ophiostoma piceae
0.98
-
triolein pH 7.0, 37°C Ophiostoma piceae
0.99
-
cholesteryl linoleate pH 7.0, 37°C Ophiostoma piceae
1
-
cholesteryl oleate pH 7.0, 37°C Ophiostoma piceae
1.1
-
cholesteryl stearate pH 7.0, 37°C Ophiostoma piceae
3
-
cholesteryl butyrate pH 7.0, 37°C Ophiostoma piceae
9.9
-
tributyrin pH 7.0, 37°C Ophiostoma piceae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Ophiostoma piceae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56500
-
x * 56500, SDS-PAGE Ophiostoma piceae

Organism

Organism UniProt Comment Textmining
Ophiostoma piceae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme contains 8% N-linked carbohydrate Ophiostoma piceae

Purification (Commentary)

Purification (Comment) Organism
2.4fold Ophiostoma piceae

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Ophiostoma piceae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
121
-
purified enzyme Ophiostoma piceae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
Ophiostoma piceae 4-nitrophenol + butyrate
-
?
4-nitrophenyl palmitate + H2O
-
Ophiostoma piceae 4-nitrophenol + palmitate
-
?
cholesteryl butyrate + H2O
-
Ophiostoma piceae cholesterol + butyrate
-
?
cholesteryl linoleate + H2O
-
Ophiostoma piceae cholesterol + linoleate
-
?
cholesteryl oleate + H2O
-
Ophiostoma piceae cholesterol + oleate
-
?
cholesteryl palmitate + H2O
-
Ophiostoma piceae cholesterol + palmitate
-
?
cholesteryl stearate + H2O
-
Ophiostoma piceae cholesterol + stearate
-
?
additional information no activity with triacetin, enzyme activity increases with increasing chain lebgth and number of double bonds, no interfacial activation Ophiostoma piceae ?
-
?
tributyrin + H2O
-
Ophiostoma piceae dibutyrin + butyrate
-
?
triolein + H2O best substrate Ophiostoma piceae diolein + oleate
-
?

Subunits

Subunits Comment Organism
? x * 56500, SDS-PAGE Ophiostoma piceae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Ophiostoma piceae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
24 h, loss of 20% activity Ophiostoma piceae
45
-
24 h, loss of 75% activity Ophiostoma piceae
60
-
inactivation Ophiostoma piceae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
44
-
4-nitrophenyl butyrate pH 7.0, 37°C Ophiostoma piceae
47
-
cholesteryl butyrate pH 7.0, 37°C Ophiostoma piceae
67
-
cholesteryl palmitate pH 7.0, 37°C Ophiostoma piceae
71
-
cholesteryl stearate pH 7.0, 37°C Ophiostoma piceae
74
-
4-nitrophenyl palmitate pH 7.0, 37°C Ophiostoma piceae
138
-
cholesteryl oleate pH 7.0, 37°C Ophiostoma piceae
150
-
cholesteryl linoleate pH 7.0, 37°C Ophiostoma piceae
179
-
tributyrin pH 7.0, 37°C Ophiostoma piceae
290
-
triolein pH 7.0, 37°C Ophiostoma piceae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 substrate 4-nitrophenyl butyrate Ophiostoma piceae

pI Value

Organism Comment pI Value Maximum pI Value
Ophiostoma piceae isoelectric focusing
-
3.3