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Literature summary for 3.1.1.13 extracted from
- Purification and characterization of cholesterol esterase from porcine pancreas (1977), Biochim. Biophys. Acta, 486, 103-113.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropylfluorophosphate | complete inhibition above 0.1 mM | Sus scrofa |  |
| p-mercuribenzoate | 15% and 80% inhibition at 1 and 8.3 mM, partially reversed by addition of DTT | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | 82000 | gel filtration, SDS-PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | Sus scrofa | - |
cholesterol + fatty acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 94% purity, chromatography techniques | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.17 | - |
for p-nitrophenyl acetate | Sus scrofa |
| 42.5 | - |
for cholesteryl oleate | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Sus scrofa | 4-nitrophenol + acetate | - |
? | |
| cholesteryl esters + H2O | - |
Sus scrofa | cholesterol + fatty acid | - |
? | |
| cholesteryl oleate + H2O | - |
Sus scrofa | cholesterol + oleic acid | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Sus scrofa | p-nitrophenol + acetic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 79000-82000, SDS-PAGE and gel filtration | Sus scrofa |
| multimer | complex of monomers | Sus scrofa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
cholesteryl oleate | - |
Sus scrofa | |
| 1.07 | - |
4-nitrophenyl acetate | - |
Sus scrofa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Sus scrofa |
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