Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cholic acid | other bile-salts not effective, form an enzymatically active hexamer | Rattus sp. |
General Stability | Organism |
---|---|
more stable in aggregates than in monomers | Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl esters + H2O | Rattus sp. | - |
cholesterol + fatty acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
500fold, standard chromatography techniques to homogeneity | Rattus sp. |
digitonin-solubilized, affinity chromatography to homogeneity | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.5 | - |
purified by affinity chromatography | Rattus sp. |
43.5 | - |
purified by standard chromatography techniques | Rattus sp. |
Storage Stability | Organism |
---|---|
-20°C, enzyme aggregates, 10 months | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl esters + H2O | - |
Rattus sp. | cholesterol + fatty acid | - |
? | |
cholesteryl oleate + H2O | - |
Rattus sp. | cholesterol + oleic acid | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
for esterification | Rattus sp. |
6.6 | - |
for hydrolysis | Rattus sp. |