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Literature summary for 3.1.1.13 extracted from

  • Gallo, L.L.
    Sterol ester hydrolase from rat pancreas (1981), Methods Enzymol., 71, 664-674.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cholic acid other bile-salts not effective, form an enzymatically active hexamer Rattus sp.

General Stability

General Stability Organism
more stable in aggregates than in monomers Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
SDS-PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesteryl esters + H2O Rattus sp.
-
cholesterol + fatty acid
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
500fold, standard chromatography techniques to homogeneity Rattus sp.
digitonin-solubilized, affinity chromatography to homogeneity Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.5
-
purified by affinity chromatography Rattus sp.
43.5
-
purified by standard chromatography techniques Rattus sp.

Storage Stability

Storage Stability Organism
-20°C, enzyme aggregates, 10 months Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl esters + H2O
-
Rattus sp. cholesterol + fatty acid
-
?
cholesteryl oleate + H2O
-
Rattus sp. cholesterol + oleic acid
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
for esterification Rattus sp.
6.6
-
for hydrolysis Rattus sp.