Literature summary for 3.1.1.112 extracted from

Fukuda, K.; Kiyokawa, Y.; Yanagiuchi, T.; Wakai, Y.; Kitamoto, K.; Inoue, Y.; Kimura, A.
Purification and characterization of isoamyl acetate-hydrolyzing esterase encoded by the IAH1 gene of Saccharomyces cerevisiae from a recombinant Escherichia coli (2000), Appl. Microbiol. Biotechnol., 53, 596-600 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	1 mM, 143% of initial activity	Saccharomyces cerevisiae
dithiothreitol	1 mM, 160% of initial activity	Saccharomyces cerevisiae
EDTA	1 mM, 133% of initial activity	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
4-chloromercuribenzoate	0.1 mM, 40% residual activity	Saccharomyces cerevisiae
diisopropyl fluorophosphate	0.1 mM, no residual activity	Saccharomyces cerevisiae
Hg2+	1 mM, no residual activity	Saccharomyces cerevisiae
iodoacetamide	1 mM, no residual activity	Saccharomyces cerevisiae
Triton X-100	1%, 72% residual activtiy	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
15.3	-	2-methylpropyl acetate	pH 7.0, 25°C	Saccharomyces cerevisiae
40.3	-	3-methylbutyl acetate	pH 7.0, 25°C	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P41734	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-methylpropyl acetate + H2O	-	Saccharomyces cerevisiae	2-methylpropanol + acetate	-	?
3-methylbutyl acetate + H2O	about 80% of the activity with 2-methylpropyl acetate	Saccharomyces cerevisiae	3-methylbutanol + acetate	-	?
isopropyl acetate + H2O	about 70% of the activity with 2-methylpropyl acetate	Saccharomyces cerevisiae	isopropanol + acetate	-	?
N-propyl acetate + H2O	about 85% of the activity with 2-methylpropyl acetate	Saccharomyces cerevisiae	n-propanol + acetate	-	?

Subunits

Subunits	Comment	Organism
?	x * 28000, SDS-PAGE, x * 37305, calculated from sequence	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
IAH1	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)