Cloned (Comment) | Organism |
---|---|
gene pmt, quantitative realtime PCR expression analysis | Xanthomonas oryzae pv. oryzae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a enzyme-deficient pmt- mutant strain by gene disruption. A complementing strain (pmt-/pHM1+ pmt) expressing the Pmt protein from the overexpression vector pHM1 restored the pectin deesterifying activity and thus formed a red zone like wild-type strain | Xanthomonas oryzae pv. oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Xanthomonas oryzae pv. oryzae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + n H2O | Xanthomonas oryzae pv. oryzae | - |
n methanol + pectate | - |
? | |
pectin + n H2O | Xanthomonas oryzae pv. oryzae KACC10331 | - |
n methanol + pectate | - |
? | |
pectin + n H2O | Xanthomonas oryzae pv. oryzae KXO85 | - |
n methanol + pectate | - |
? | |
pectin + n H2O | Xanthomonas oryzae pv. oryzae BXO43 | - |
n methanol + pectate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas oryzae pv. oryzae | Q5GZC1 | - |
- |
Xanthomonas oryzae pv. oryzae BXO43 | Q5GZC1 | - |
- |
Xanthomonas oryzae pv. oryzae KACC10331 | Q5GZC1 | - |
- |
Xanthomonas oryzae pv. oryzae KXO85 | Q5GZC1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no difference in the expression of pmt gene by laboratory wild-type Xoo strain (BXO43) grown in either nutrient rich PS medium or in plant mimic XOM2 medium | Xanthomonas oryzae pv. oryzae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + n H2O | - |
Xanthomonas oryzae pv. oryzae | n methanol + pectate | - |
? | |
pectin + n H2O | - |
Xanthomonas oryzae pv. oryzae KACC10331 | n methanol + pectate | - |
? | |
pectin + n H2O | - |
Xanthomonas oryzae pv. oryzae KXO85 | n methanol + pectate | - |
? | |
pectin + n H2O | - |
Xanthomonas oryzae pv. oryzae BXO43 | n methanol + pectate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pectin methyl esterase | - |
Xanthomonas oryzae pv. oryzae |
pectin methylesterase | - |
Xanthomonas oryzae pv. oryzae |
PemB | UniProt | Xanthomonas oryzae pv. oryzae |
PMT | - |
Xanthomonas oryzae pv. oryzae |
Xoo | - |
Xanthomonas oryzae pv. oryzae |
XOO2696 | gene name, UniProt | Xanthomonas oryzae pv. oryzae |
General Information | Comment | Organism |
---|---|---|
malfunction | mutations in the pglA, pmt, pel and pelL genes have minimal effects on virulence | Xanthomonas oryzae pv. oryzae |
physiological function | presence of four pectin homogalacturonan (HG) degrading genes in the genome of Xoo. The four HG degrading genes include one polygalacturonase (pglA), one pectin methyl esterase (pmt) and two pectate lyases (pel and pelL). PglA is the major pectin degrading enzyme produced by Xoo. The pectin methyl esterase, Pmt, is the pectin deesterifying enzyme secreted by Xoo as evident from the enzymatic activity assay performed using pectin as the substrate. Compared to cellulases and xylanases, the HG degrading enzymes may not have a major role in the pathogenicity of strain BXO43 | Xanthomonas oryzae pv. oryzae |