Application | Comment | Organism |
---|---|---|
food industry | due to very high de-esterification activity, easy denaturation and significant efficacy in incrementing clarification of fruit juice makes the enzyme useful for industrial application | Datura inoxia |
food industry | due to very high de-esterification activity, easy denaturation and significant efficacy in incrementing clarification of fruit juice makes the enzyme useful for industrial application | Datura stramonium |
food industry | due to very high de-esterification activity, easy denaturation and significant efficacy in incrementing clarification of fruit juice makes the enzyme useful for industrial application | Datura metel |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | optimum activity in the presence of 0.3 M NaCl | Datura inoxia | |
NaCl | optimum activity in the presence of 0.3 M NaCl | Datura stramonium | |
NaCl | optimum activity in the presence of 0.3 M NaCl | Datura metel |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Datura inoxia |
33000 | - |
x * 33000, SDS-PAGE | Datura stramonium |
33000 | - |
x * 33000, SDS-PAGE | Datura metel |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | Datura inoxia | - |
methanol + pectate | - |
? | |
pectin + H2O | Datura stramonium | - |
methanol + pectate | - |
? | |
pectin + H2O | Datura metel | - |
methanol + pectate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Datura inoxia | - |
- |
- |
Datura metel | - |
- |
- |
Datura stramonium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 200 gel filtration | Datura inoxia |
ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 200 gel filtration | Datura stramonium |
ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 200 gel filtration | Datura metel |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
exocarp | highest activity | Datura inoxia | - |
exocarp | highest activity | Datura stramonium | - |
exocarp | highest activity | Datura metel | - |
leaf | - |
Datura inoxia | - |
leaf | - |
Datura stramonium | - |
leaf | - |
Datura metel | - |
seed | - |
Datura inoxia | - |
seed | - |
Datura stramonium | - |
seed | - |
Datura metel | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Datura inoxia | methanol + pectate | - |
? | |
pectin + H2O | - |
Datura stramonium | methanol + pectate | - |
? | |
pectin + H2O | - |
Datura metel | methanol + pectate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Datura inoxia |
? | x * 33000, SDS-PAGE | Datura stramonium |
? | x * 33000, SDS-PAGE | Datura metel |
Synonyms | Comment | Organism |
---|---|---|
pectin methylesterase | - |
Datura inoxia |
pectin methylesterase | - |
Datura stramonium |
pectin methylesterase | - |
Datura metel |
PME | - |
Datura inoxia |
PME | - |
Datura stramonium |
PME | - |
Datura metel |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Datura inoxia |
60 | - |
- |
Datura stramonium |
60 | - |
- |
Datura metel |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | maximum activity is observed at 60°C after that activity decreases sharply up to almost zero at 90°C | Datura inoxia |
60 | 90 | maximum activity is observed at 60°C. The activity decreases sharply up to almost zero at 90°C | Datura stramonium |
60 | 90 | maximum activity is observed at 60°C. The activity decreases sharply up to almost zero at 90°C | Datura metel |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | the enzyme retains more than 90% activity at 60°C for 60 min. At 70°C, the enzyme loses 46 and 61% activity in 30 and 60 min, respectively. Activity is completely abolished at 80°C after 5 min of incubation | Datura inoxia |
60 | 80 | the enzyme retains more than 90% activity at 60°C for 60 min. At 70°C, the enzyme loses 46 and 61% activity in 30 and 60 min, respectively. Activity is completely abolished at 80°C after 5 min of incubation | Datura stramonium |
60 | 80 | the enzyme retains more than 90% activity at 60°C for 60 min. At 70°C, the enzyme loses 46 and 61% activity in 30 and 60 min, respectively. Activity is completely abolished at 80°C after 5 min of incubation | Datura metel |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Datura inoxia |
9 | - |
- |
Datura stramonium |
9 | - |
- |
Datura metel |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | high activity is observed between pH 7.0 and 10.0 | Datura inoxia |
7 | 10 | high activity is observed between pH 7.0 and 10.0 | Datura stramonium |
7 | 10 | high activity is observed between pH 7.010.0 | Datura metel |