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Literature summary for 3.1.1.11 extracted from

  • Jiang, X.; Chen, P.; Yin, M.; Yang, Q.
    Constitutive expression, purification and characterisation of pectin methylesterase from Aspergillus niger in Pichia pastoris for potential application in the fruit juice industry (2013), J. Sci. Food Agric., 93, 375-381.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Aspergillus niger

Protein Variants

Protein Variants Comment Organism
food industry fruit juice industry Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 52% residual activity at 100 mM Aspergillus niger
K+ 73% residual activity at 100 mM Aspergillus niger
Mg2+ 86% residual activity at 100 mM Aspergillus niger
Mn2+ 61% residual activity at 100 mM Aspergillus niger
Ni2+ 47% residual activity at 100 mM Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Na+ Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectin + H2O Aspergillus niger
-
methanol + pectate
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, HiTrap Q column chromatography, and Resource S column chromatography Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.67
-
supernatant, at pH 4.7 and 50°C Aspergillus niger
342
-
after 35.4fold purification, at pH 4.7 and 50°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
Aspergillus niger methanol + pectate
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
pectin methylesterase
-
Aspergillus niger
PME
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 90-100% activity from 35-50°C, about 30% activity at 55°C, no activity at 60°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
-
Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
4.5 5 about 90% activity at pH 4.5,4.6, and 4.8, 100% activity at pH 4.7, about 60% activity at pH 4.9, about 50% activity at pH 5.0 Aspergillus niger