Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM101, IPTG induction at 37°C instead of 25-28°C results in approximately 10times less PME activity in the extract | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | a purified kiwi (Actinidia chinensis) pectin methylesterase inhibitor has no effect on the activity of the enzyme | Arabidopsis thaliana | |
NaCl | activity decreases in the presence of 0.1 M NaCl and is 4times lower in 0.5 M NaCl | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require salt for activity | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Ni-NTA agarose column chromatography | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.246 | - |
crude extract | Arabidopsis thaliana |
11.98 | - |
purified enzyme | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-20°C, crude extract, several weeks, no significant loss of activity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Arabidopsis thaliana | methanol + pectate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pectin methylesterase | - |
Arabidopsis thaliana |
PME31 | - |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | incubation at 30°C has little but significant effect on enzyme activity, the incubation at 55°C abolishes over 95% of enzyme activity | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | in phosphate buffer | Arabidopsis thaliana |
9 | - |
around pH 9 in glycine buffer | Arabidopsis thaliana |