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Literature summary for 3.1.1.11 extracted from

  • Arotupin, D.J.; Akinyosoye, F.A.; Onifade, A.K.
    Purification and characterization of pectin methylesterase from Aspergillus repens isolated from cultivated soil (2008), Afr. J. Biotechnol., 7, 1991-1998.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Aspergillus pseudoglaucus
HgCl2 1.0 mM of HgCl2 results in approximately 50% loss of enzyme activity, while concentration of 8 mM produces complete loss of enzyme activity Aspergillus pseudoglaucus
iodoacetic acid
-
Aspergillus pseudoglaucus
PbCl2
-
Aspergillus pseudoglaucus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the highest PME activity occurrs at 20 mM of Ca2+, further increase in concentration results in the decline of the enzyme activity Aspergillus pseudoglaucus
K+ the activity of the enzyme increases with increase in the concentration of K+, further increase in concentration results in the decline of the enzyme activity Aspergillus pseudoglaucus
Mg2+ highest activity at 15 mM Mg2+, further increase in concentration results in the decline of the enzyme activity Aspergillus pseudoglaucus
Na+ the activity of the enzyme increases with increase in the concentration of Na+, further increase in concentration results in the decline of the enzyme activity Aspergillus pseudoglaucus
Zn2+ highest activity at 15 mM Zn2+, further increase in concentration results in the decline of the enzyme activity Aspergillus pseudoglaucus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
141300
-
gel filtration Aspergillus pseudoglaucus

Organism

Organism UniProt Comment Textmining
Aspergillus pseudoglaucus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex G-100 gel filtration, and Sephadex C-50 gel filtration Aspergillus pseudoglaucus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.82
-
crude extract Aspergillus pseudoglaucus
6.5
-
after 7.93fold purification Aspergillus pseudoglaucus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
Aspergillus pseudoglaucus methanol + pectate
-
?

Synonyms

Synonyms Comment Organism
pectin methylesterase
-
Aspergillus pseudoglaucus
PME
-
Aspergillus pseudoglaucus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Aspergillus pseudoglaucus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
the optimum temperature for the activity of pectimethylesterase is 30°C, however, within 10 min of heating approximately 50% loss of enzyme activity is lost, additional 10 min of heating results in retention of only 9% enzyme activity, and further heating results in complete loss of enzyme activity Aspergillus pseudoglaucus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Aspergillus pseudoglaucus