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Literature summary for 3.1.1.11 extracted from
- Purification and thermal and high-pressure inactivation of pectinmethylesterase isoenzymes from tomatoes (Lycopersicon esculentum): a novel pressure labile isoenzyme (2007), J. Agric. Food Chem., 55, 9259-9265.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PMEI | PME inhibitor | Solanum lycopersicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Solanum lycopersicum | - |
methanol + pectate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
four isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozymes about 12fold by ammonium sulfate fractionation, PME inhibitor PMEI affinity chromatography, and cation exchange chromatography | Solanum lycopersicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 834 | 847 | purified isozymes | Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | - |
Solanum lycopersicum | methanol + pectate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34500-35000, four isozymes, SDS-PAGE | Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pectin methylesterase | - |
Solanum lycopersicum |
| PME | - |
Solanum lycopersicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Solanum lycopersicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | - |
Solanum lycopersicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal and high-pressure inactivation kinetics of the two major isoenzymes, a thermolabile and a thermostable one, inactivation kinetics at pH 6.0 are accurately described by a first-order model, overview, the thermostable isoenzyme is pressure-stable, overview | Solanum lycopersicum |
| 60 | - |
5 min, thermolabile isozyme, complete inactivation | Solanum lycopersicum |
| 70 | - |
5 min, thermostable isozyme, complete inactivation | Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Solanum lycopersicum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Solanum lycopersicum |
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