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Literature summary for 3.1.1.11 extracted from

  • Duvetter, T.; Fraeye, I.; Sila, D.N.; Verlent, I.; Smout, C.; Hendrickx, M.; Van Loey, A.
    Mode of de-esterification of alkaline and acidic pectin methyl esterases at different pH conditions (2006), J. Agric. Food Chem., 54, 7825-7831.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectin + H2O Solanum lycopersicum
-
methanol + pectate
-
?
pectin + H2O Aspergillus aculeatus
-
methanol + pectate
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus
-
-
-
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
further purification of a commercial preparation by gel filtration Aspergillus aculeatus
native enzyme partially from fruits by ammonium sulfate fractionation and dialysis Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation recombinant enzyme Aspergillus aculeatus
-
fruit
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
Solanum lycopersicum methanol + pectate
-
?
pectin + H2O
-
Aspergillus aculeatus methanol + pectate
-
?
pectin + H2O citrus pectin Solanum lycopersicum methanol + pectate
-
?
pectin + H2O citrus pectin Aspergillus aculeatus methanol + pectate
-
?

Synonyms

Synonyms Comment Organism
pectin methyl esterase
-
Solanum lycopersicum
pectin methyl esterase
-
Aspergillus aculeatus
PME
-
Solanum lycopersicum
PME
-
Aspergillus aculeatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Solanum lycopersicum
22
-
assay at Aspergillus aculeatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
5 min, partially purified enzyme, inactivation Solanum lycopersicum
80
-
5 min, purified enzyme, inactivation Aspergillus aculeatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
acidic pH optimum Aspergillus aculeatus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 pH-dependent activity pattern, pH-profile, overview Solanum lycopersicum
4.5 8 pH-dependent activity pattern, pH-profile, overview Aspergillus aculeatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus aculeatus acidic isoelectric pH
-
additional information