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Literature summary for 3.1.1.11 extracted from
- A pectin methylesterase as an allergenic marker for the sensitization to Russian thistle (Salsola kali) pollen (2007), Clin. Exp. Allergy, 37, 1111-1119.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | pectin methylesterase is an allergenic marker for the sensitization to Russian thistle, Salsola kali pollen | Kali turgidum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Kali turgidum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37214 | - |
x * 40000, native enzyme, SDS-PAGE, x * 37214, native glycosylated enzyme, mass spectrometry | Kali turgidum |
| 40000 | - |
x * 40000, native enzyme, SDS-PAGE, x * 37214, native glycosylated enzyme, mass spectrometry | Kali turgidum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Kali turgidum | the mildly basic and polymorphic protein causes allergic reactions in humans determined by secific IgE production | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kali turgidum | Q17ST3 | frgament | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate detection, overview | Kali turgidum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from pollen by gel filtration, anion exchange chromatography and isoelectric focusing | Kali turgidum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| pectin + n H2O = n methanol + pectate | the catalytic residues Gln151, Gln173, Asp174, Asp195, and Arg253 are conserved in Sal k 1 | Kali turgidum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pollen | - |
Kali turgidum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the mildly basic and polymorphic protein causes allergic reactions in humans determined by secific IgE production | Kali turgidum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, native enzyme, SDS-PAGE, x * 37214, native glycosylated enzyme, mass spectrometry | Kali turgidum |
| More | model of the three-dimensional structure with conserved parallel beta-sheet coiled into a large, right-handed cylinder based on the structure of the Erwinia chrysantemi enzyme with PDB ID 1QJV, overview | Kali turgidum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the plant pectin methylesterase family | Kali turgidum |
| pectine methylesterase | - |
Kali turgidum |
| PME | - |
Kali turgidum |
| Sal k 1 | - |
Kali turgidum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Kali turgidum | due to heterogenities | 7.8 | 6.7 |
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Release 2023.1 (January 2023)
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