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Literature summary for 3.1.1.11 extracted from
- Overproduction in Escherichia coli of the pectin methylesterase A from Erwinia chrysanthemi 3937: one-step purification, biochemical characterization, and production of polyclonal antibodies (2000), Can. J. Microbiol., 46, 474-480.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Dickeya chrysanthemi |
General Stability
| General Stability | Organism |
|---|---|
| significant loss of activity after freezing and thawing | Dickeya chrysanthemi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
citrus pectin | pH 6 and pH 7.6 | Dickeya chrysanthemi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dickeya chrysanthemi | - |
- |
- |
| Dickeya chrysanthemi 3937 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity, one-step purification | Dickeya chrysanthemi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Dickeya chrysanthemi |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme, stable for 1 week | Dickeya chrysanthemi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrus pectin + H2O | highest activity with pectin with an esterification degree of 50% | Dickeya chrysanthemi | methanol + citrus pectate | - |
? |  |
| citrus pectin + H2O | highest activity with pectin with an esterification degree of 50% | Dickeya chrysanthemi 3937 | methanol + citrus pectate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Dickeya chrysanthemi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
no loss of activity after 20 min | Dickeya chrysanthemi |
| 55 | - |
50% loss of activity after 5 min | Dickeya chrysanthemi |
| 60 | - |
complete loss of activity after 2 min | Dickeya chrysanthemi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Dickeya chrysanthemi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | about 55% activity at pH 5-6 | Dickeya chrysanthemi |
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Release 2023.1 (January 2023)
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