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Literature summary for 3.1.1.11 extracted from
- Purification and characterization of Erwinia chrysanthemi B374 pectin methylesterase produced by Bacillus subtilis (1992), Enzyme Microb. Technol., 14, 832-836.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing results in a substantial loss of activity | Dickeya chrysanthemi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-galacturonate | slight | Dickeya chrysanthemi |  |
| Polygalacturonate | competitive | Dickeya chrysanthemi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dickeya chrysanthemi | - |
- |
- |
| Dickeya chrysanthemi B374 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Dickeya chrysanthemi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.19 | - |
- |
Dickeya chrysanthemi |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for at least 1 week | Dickeya chrysanthemi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anhydrogalacturonate + H2O | - |
Dickeya chrysanthemi | ? | - |
? | |
| anhydrogalacturonate + H2O | - |
Dickeya chrysanthemi B374 | ? | - |
? | |
| pectin + H2O | - |
Dickeya chrysanthemi | methanol + pectate | - |
? | |
| pectin + H2O | - |
Dickeya chrysanthemi B374 | methanol + pectate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Dickeya chrysanthemi |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
65 | 0°C: about 20% of maximal activity, 65°C: 10% of maximal activity | Dickeya chrysanthemi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
16 min, in absence of substrate, stable up to | Dickeya chrysanthemi |
| 50 | - |
pH 6.0, 16 min, 50% loss of activity | Dickeya chrysanthemi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Dickeya chrysanthemi |
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