Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | about 160% activity at 10 mM | Geobacillus thermodenitrificans | |
dithiothreitol | about 140% activity at 1 mM | Geobacillus thermodenitrificans | |
EDTA | about 125% activity at 1 mM | Geobacillus thermodenitrificans | |
n-hexane | about 140% activity at 30% (v/v) | Geobacillus thermodenitrificans | |
Triton X-100 | about 150% activity at 10 mM | Geobacillus thermodenitrificans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) One Shot cells | Geobacillus thermodenitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | about 60% residual activity at 30% (v/v) | Geobacillus thermodenitrificans | |
acetonitrile | about 30% residual activity at 10% (v/v) | Geobacillus thermodenitrificans | |
chloroform | about 40% residual activity at 30% (v/v) | Geobacillus thermodenitrificans | |
Cu2+ | about 70% residual activity at 10 mM | Geobacillus thermodenitrificans | |
dimethylformamide | about 50% residual activity at 10% (v/v) | Geobacillus thermodenitrificans | |
DMSO | about 60% residual activity at 30% (v/v) | Geobacillus thermodenitrificans | |
ethanol | about 40% residual activity at 30% (v/v) | Geobacillus thermodenitrificans | |
Fe2+ | about 70% residual activity at 10 mM | Geobacillus thermodenitrificans | |
K+ | about 70% residual activity at 10 mM | Geobacillus thermodenitrificans | |
methanol | about 50% residual activity at 30% (v/v) | Geobacillus thermodenitrificans | |
additional information | EDTA has no inhibiting effect | Geobacillus thermodenitrificans | |
n-butanol | about 30% residual activity at 10% (v/v) | Geobacillus thermodenitrificans | |
phenylmethylsulfonyl fluoride | about 25% residual activity at 1 mM | Geobacillus thermodenitrificans | |
propan-2-ol | about 30% residual activity at 10% (v/v) | Geobacillus thermodenitrificans | |
SDS | about 60% residual activity at 10 mM | Geobacillus thermodenitrificans | |
Zn2+ | about 70% residual activity at 10 mM | Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
4-nitrophenyl caprylate | at pH 7.5 and 55°C | Geobacillus thermodenitrificans | |
0.64 | - |
4-nitrophenyl butyrate | at pH 7.5 and 55°C | Geobacillus thermodenitrificans | |
0.97 | - |
4-nitrophenyl acetate | at pH 7.5 and 55°C | Geobacillus thermodenitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | about 110% activity at 10 mM | Geobacillus thermodenitrificans | |
additional information | not influenced by Ca2+ and Mg2+ | Geobacillus thermodenitrificans | |
Na+ | about 110% activity at 10 mM | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
gel filtration | Geobacillus thermodenitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
iron(III)-enterobactin + 3 H2O | Geobacillus thermodenitrificans | overall reaction | iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine | - |
? | |
iron(III)-enterobactin + 3 H2O | Geobacillus thermodenitrificans NG80-2 | overall reaction | iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine | - |
? | |
iron(III)-enterobactin + H2O | Geobacillus thermodenitrificans | - |
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + N-(2,3-dihydroxybenzoyl)-L-serine | - |
? | |
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + H2O | Geobacillus thermodenitrificans | - |
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + N-(2,3-dihydroxybenzoyl)-L-serine | - |
? | |
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + H2O | Geobacillus thermodenitrificans | - |
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + N-(2,3-dihydroxybenzoyl)-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | A0A1W6VPA7 | - |
- |
Geobacillus thermodenitrificans NG80-2 | A0A1W6VPA7 | - |
- |
Purification (Comment) | Organism |
---|---|
His Trap column chromatography, Ni-TED 1000 column chromatography, and Superdex 75 gel filtration | Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | best substrate | Geobacillus thermodenitrificans | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | best substrate | Geobacillus thermodenitrificans NG80-2 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Geobacillus thermodenitrificans | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Geobacillus thermodenitrificans NG80-2 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Geobacillus thermodenitrificans | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Geobacillus thermodenitrificans NG80-2 | 4-nitrophenol + caprylate | - |
? | |
iron(III)-enterobactin + 3 H2O | overall reaction | Geobacillus thermodenitrificans | iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine | - |
? | |
iron(III)-enterobactin + 3 H2O | overall reaction | Geobacillus thermodenitrificans NG80-2 | iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine | - |
? | |
iron(III)-enterobactin + H2O | - |
Geobacillus thermodenitrificans | iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + N-(2,3-dihydroxybenzoyl)-L-serine | - |
? | |
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + H2O | - |
Geobacillus thermodenitrificans | iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + N-(2,3-dihydroxybenzoyl)-L-serine | - |
? | |
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + H2O | - |
Geobacillus thermodenitrificans | iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + N-(2,3-dihydroxybenzoyl)-L-serine | - |
? | |
additional information | no activity with 4-nitrophenyl laurate, bacillibactin and enterobactin | Geobacillus thermodenitrificans | ? | - |
? | |
additional information | no activity with 4-nitrophenyl laurate, bacillibactin and enterobactin | Geobacillus thermodenitrificans NG80-2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Geobacillus thermodenitrificans |
monomer | 1 * 25940, calculated from amino acid sequence | Geobacillus thermodenitrificans |
Synonyms | Comment | Organism |
---|---|---|
EstGtA3 | - |
Geobacillus thermodenitrificans |
GTNG_0744 | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus thermodenitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 65 | the enzyme is 100% stable when incubated for 16 h at 55°C. About 20% residual activity is observed after 5 min at 65°C | Geobacillus thermodenitrificans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.24 | - |
4-nitrophenyl caprylate | at pH 7.5 and 55°C | Geobacillus thermodenitrificans | |
3.03 | - |
4-nitrophenyl butyrate | at pH 7.5 and 55°C | Geobacillus thermodenitrificans | |
6.8 | - |
4-nitrophenyl acetate | at pH 7.5 and 55°C | Geobacillus thermodenitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | the enzyme is active in the pH range 7.0-8.5 | Geobacillus thermodenitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require cofactors for its activity | Geobacillus thermodenitrificans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.73 | - |
4-nitrophenyl butyrate | at pH 7.5 and 55°C | Geobacillus thermodenitrificans | |
7.01 | - |
4-nitrophenyl acetate | at pH 7.5 and 55°C | Geobacillus thermodenitrificans | |
248 | - |
4-nitrophenyl caprylate | at pH 7.5 and 55°C | Geobacillus thermodenitrificans |