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Literature summary for 3.1.1.108 extracted from

  • Curci, N.; Strazzulli, A.; De Lise, F.; Iacono, R.; Maurelli, L.; Dal Piaz, F.; Cobucci-Ponzano, B.; Moracci, M.
    Identification of a novel esterase from the thermophilic bacterium Geobacillus thermodenitrificans NG80-2 (2019), Extremophiles, 23, 407-419 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol about 160% activity at 10 mM Geobacillus thermodenitrificans
dithiothreitol about 140% activity at 1 mM Geobacillus thermodenitrificans
EDTA about 125% activity at 1 mM Geobacillus thermodenitrificans
n-hexane about 140% activity at 30% (v/v) Geobacillus thermodenitrificans
Triton X-100 about 150% activity at 10 mM Geobacillus thermodenitrificans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) One Shot cells Geobacillus thermodenitrificans

Inhibitors

Inhibitors Comment Organism Structure
acetone about 60% residual activity at 30% (v/v) Geobacillus thermodenitrificans
acetonitrile about 30% residual activity at 10% (v/v) Geobacillus thermodenitrificans
chloroform about 40% residual activity at 30% (v/v) Geobacillus thermodenitrificans
Cu2+ about 70% residual activity at 10 mM Geobacillus thermodenitrificans
dimethylformamide about 50% residual activity at 10% (v/v) Geobacillus thermodenitrificans
DMSO about 60% residual activity at 30% (v/v) Geobacillus thermodenitrificans
ethanol about 40% residual activity at 30% (v/v) Geobacillus thermodenitrificans
Fe2+ about 70% residual activity at 10 mM Geobacillus thermodenitrificans
K+ about 70% residual activity at 10 mM Geobacillus thermodenitrificans
methanol about 50% residual activity at 30% (v/v) Geobacillus thermodenitrificans
additional information EDTA has no inhibiting effect Geobacillus thermodenitrificans
n-butanol about 30% residual activity at 10% (v/v) Geobacillus thermodenitrificans
phenylmethylsulfonyl fluoride about 25% residual activity at 1 mM Geobacillus thermodenitrificans
propan-2-ol about 30% residual activity at 10% (v/v) Geobacillus thermodenitrificans
SDS about 60% residual activity at 10 mM Geobacillus thermodenitrificans
Zn2+ about 70% residual activity at 10 mM Geobacillus thermodenitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
4-nitrophenyl caprylate at pH 7.5 and 55°C Geobacillus thermodenitrificans
0.64
-
4-nitrophenyl butyrate at pH 7.5 and 55°C Geobacillus thermodenitrificans
0.97
-
4-nitrophenyl acetate at pH 7.5 and 55°C Geobacillus thermodenitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ about 110% activity at 10 mM Geobacillus thermodenitrificans
additional information not influenced by Ca2+ and Mg2+ Geobacillus thermodenitrificans
Na+ about 110% activity at 10 mM Geobacillus thermodenitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
gel filtration Geobacillus thermodenitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
iron(III)-enterobactin + 3 H2O Geobacillus thermodenitrificans overall reaction iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine
-
?
iron(III)-enterobactin + 3 H2O Geobacillus thermodenitrificans NG80-2 overall reaction iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine
-
?
iron(III)-enterobactin + H2O Geobacillus thermodenitrificans
-
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
?
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + H2O Geobacillus thermodenitrificans
-
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
?
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + H2O Geobacillus thermodenitrificans
-
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans A0A1W6VPA7
-
-
Geobacillus thermodenitrificans NG80-2 A0A1W6VPA7
-
-

Purification (Commentary)

Purification (Comment) Organism
His Trap column chromatography, Ni-TED 1000 column chromatography, and Superdex 75 gel filtration Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O best substrate Geobacillus thermodenitrificans 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O best substrate Geobacillus thermodenitrificans NG80-2 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Geobacillus thermodenitrificans 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Geobacillus thermodenitrificans NG80-2 4-nitrophenol + butyrate
-
?
4-nitrophenyl caprylate + H2O
-
Geobacillus thermodenitrificans 4-nitrophenol + caprylate
-
?
4-nitrophenyl caprylate + H2O
-
Geobacillus thermodenitrificans NG80-2 4-nitrophenol + caprylate
-
?
iron(III)-enterobactin + 3 H2O overall reaction Geobacillus thermodenitrificans iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine
-
?
iron(III)-enterobactin + 3 H2O overall reaction Geobacillus thermodenitrificans NG80-2 iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + 2 N-(2,3-dihydroxybenzoyl)-L-serine
-
?
iron(III)-enterobactin + H2O
-
Geobacillus thermodenitrificans iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
?
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + H2O
-
Geobacillus thermodenitrificans iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
?
iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine trimer complex + H2O
-
Geobacillus thermodenitrificans iron(III)-N-(2,3-dihydroxybenzoyl)-L-serine dimer complex + N-(2,3-dihydroxybenzoyl)-L-serine
-
?
additional information no activity with 4-nitrophenyl laurate, bacillibactin and enterobactin Geobacillus thermodenitrificans ?
-
?
additional information no activity with 4-nitrophenyl laurate, bacillibactin and enterobactin Geobacillus thermodenitrificans NG80-2 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Geobacillus thermodenitrificans
monomer 1 * 25940, calculated from amino acid sequence Geobacillus thermodenitrificans

Synonyms

Synonyms Comment Organism
EstGtA3
-
Geobacillus thermodenitrificans
GTNG_0744
-
Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Geobacillus thermodenitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 65 the enzyme is 100% stable when incubated for 16 h at 55°C. About 20% residual activity is observed after 5 min at 65°C Geobacillus thermodenitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.24
-
4-nitrophenyl caprylate at pH 7.5 and 55°C Geobacillus thermodenitrificans
3.03
-
4-nitrophenyl butyrate at pH 7.5 and 55°C Geobacillus thermodenitrificans
6.8
-
4-nitrophenyl acetate at pH 7.5 and 55°C Geobacillus thermodenitrificans

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 the enzyme is active in the pH range 7.0-8.5 Geobacillus thermodenitrificans

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme does not require cofactors for its activity Geobacillus thermodenitrificans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.73
-
4-nitrophenyl butyrate at pH 7.5 and 55°C Geobacillus thermodenitrificans
7.01
-
4-nitrophenyl acetate at pH 7.5 and 55°C Geobacillus thermodenitrificans
248
-
4-nitrophenyl caprylate at pH 7.5 and 55°C Geobacillus thermodenitrificans