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Literature summary for 3.1.1.106 extracted from

  • Chen, D.; Vollmar, M.; Rossi, M.N.; Phillips, C.; Kraehenbuehl, R.; Slade, D.; Mehrotra, P.V.; von Delft, F.; Crosthwaite, S.K.; Gileadi, O.; Denu, J.M.; Ahel, I.
    Identification of macrodomain proteins as novel O-acetyl-ADP-ribose deacetylases (2011), J. Biol. Chem., 286, 13261-13271 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.106

Cloned(Commentary)

Cloned (Comment) Organism
enzyme isoform MacroD1 is expressed in Escherichia coli BL21(DE3)-R3-pRARE2 cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
isoform MacroD1, sitting drop vapor diffusion method, using 0.2 M (NH4)2SO4, 0.1 M HEPES pH 7.5, 25% (w/v) PEG 3350 at 4°C Homo sapiens

Protein Variants

Protein Variants Comment Organism
C199A the mutant shows about 90.4% of wild type activity Homo sapiens
D122A the mutant shows about 24.5% of wild type activity Staphylococcus aureus
D160A the mutant shows about 57% of wild type activity Homo sapiens
D167A the mutant shows about 68.7% of wild type activity Homo sapiens
D184A the mutant shows about 39.3% of wild type activity Homo sapiens
G124E the mutant shows about 1.0% of wild type activity Escherichia coli
G214E the mutant shows about 0.2% of wild type activity Staphylococcus aureus
G270E the mutant shows about 1% of wild type activity Homo sapiens
H188A the mutant shows about 57.6% of wild type activity Homo sapiens
N171A the mutant shows about 41.3% of wild type activity Homo sapiens
N174A the mutant shows about 57.3% of wild type activity Homo sapiens
N174A/D184A the mutant shows about 7% of wild type activity Homo sapiens
S176A the mutant shows about 98.4% of wild type activity Homo sapiens
S268A the mutant shows about 77% of wild type activity Homo sapiens
T226A the mutant shows about 102.9% of wild type activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP-D-ribose
-
 Escherichia coli
ADP-D-ribose
-
 Homo sapiens
ADP-D-ribose
-
 Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.107
-
 O-acetyl-ADP-D-ribose isoform MacroD2, at pH 7.3 and 23°C Homo sapiens
0.27
-
 O-acetyl-ADP-D-ribose at pH 7.3 and 23°C Escherichia coli
0.375
-
 O-acetyl-ADP-D-ribose isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
2
-
 O-acetyl-ADP-D-ribose at pH 7.3 and 23°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2''-O-acetyl-ADP-D-ribose + H2O Staphylococcus aureus
-
 ADP-D-ribose + acetate
-
 ?
2''-O-acetyl-ADP-D-ribose + H2O Escherichia coli
-
 ADP-D-ribose + acetate
-
 ?
2''-O-acetyl-ADP-D-ribose + H2O Homo sapiens
-
 ADP-D-ribose + acetate
-
 ?
3''-O-acetyl-ADP-D-ribose + H2O Staphylococcus aureus
-
 ADP-D-ribose + acetate
-
 ?
3''-O-acetyl-ADP-D-ribose + H2O Escherichia coli
-
 ADP-D-ribose + acetate
-
 ?
3''-O-acetyl-ADP-D-ribose + H2O Homo sapiens
-
 ADP-D-ribose + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens A1Z1Q3
-
-
Homo sapiens Q9BQ69
-
-
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 75 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2''-O-acetyl-ADP-D-ribose + H2O
-
 Staphylococcus aureus ADP-D-ribose + acetate
-
 ?
2''-O-acetyl-ADP-D-ribose + H2O
-
 Escherichia coli ADP-D-ribose + acetate
-
 ?
2''-O-acetyl-ADP-D-ribose + H2O
-
 Homo sapiens ADP-D-ribose + acetate
-
 ?
3''-O-acetyl-ADP-D-ribose + H2O
-
 Staphylococcus aureus ADP-D-ribose + acetate
-
 ?
3''-O-acetyl-ADP-D-ribose + H2O
-
 Escherichia coli ADP-D-ribose + acetate
-
 ?
3''-O-acetyl-ADP-D-ribose + H2O
-
 Homo sapiens ADP-D-ribose + acetate
-
 ?
O-acetyl-ADP-D-ribose + H2O
-
 Staphylococcus aureus ADP-D-ribose + acetate
-
 ?
O-acetyl-ADP-D-ribose + H2O
-
 Escherichia coli ADP-D-ribose + acetate
-
 ?
O-acetyl-ADP-D-ribose + H2O
-
 Homo sapiens ADP-D-ribose + acetate
-
 ?

Synonyms

Synonyms Comment Organism
MacroD-like protein
-
 Staphylococcus aureus
MACROD1
-
 Homo sapiens
MacroD2
-
 Homo sapiens
O-acetyl-ADP-ribose deacetylase
-
 Staphylococcus aureus
O-acetyl-ADP-ribose deacetylase
-
 Escherichia coli
O-acetyl-ADP-ribose deacetylase
-
 Homo sapiens
OAADPr deacetylase
-
 Staphylococcus aureus
OAADPr deacetylase
-
 Escherichia coli
OAADPr deacetylase
-
 Homo sapiens
SAV0325
-
 Staphylococcus aureus
ymdB
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 O-acetyl-ADP-D-ribose mutant G270E of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.005
-
 O-acetyl-ADP-D-ribose mutant G124E, at pH 7.3 and 23°C Escherichia coli
0.007
-
 O-acetyl-ADP-D-ribose mutant G214E, at pH 7.3 and 23°C Staphylococcus aureus
0.014
-
 O-acetyl-ADP-D-ribose mutant N174A/D184A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.078
-
 O-acetyl-ADP-D-ribose mutant D184A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.082
-
 O-acetyl-ADP-D-ribose mutant N171A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.113
-
 O-acetyl-ADP-D-ribose mutant N174A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.114
-
 O-acetyl-ADP-D-ribose mutant D160A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.114
-
 O-acetyl-ADP-D-ribose mutant H188A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.12
-
 O-acetyl-ADP-D-ribose isoform MacroD2, at pH 7.3 and 23°C Homo sapiens
0.136
-
 O-acetyl-ADP-D-ribose mutant D167A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.153
-
 O-acetyl-ADP-D-ribose mutant S268A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.179
-
 O-acetyl-ADP-D-ribose mutant C199A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.195
-
 O-acetyl-ADP-D-ribose mutant S176A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.198
-
 O-acetyl-ADP-D-ribose isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.204
-
 O-acetyl-ADP-D-ribose mutant T226A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
0.48
-
 O-acetyl-ADP-D-ribose wild type enzyme, at pH 7.3 and 23°C Escherichia coli
0.679
-
 O-acetyl-ADP-D-ribose mutant D122A, at pH 7.3 and 23°C Staphylococcus aureus
2.77
-
 O-acetyl-ADP-D-ribose wild type enzyme, at pH 7.3 and 23°C Staphylococcus aureus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.145
-
 ADP-D-ribose isoform MacroD1, at pH 7.3 and 23°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.533
-
 O-acetyl-ADP-D-ribose isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
1.1
-
 O-acetyl-ADP-D-ribose isoform MacroD2, at pH 7.3 and 23°C Homo sapiens
1.78
-
 O-acetyl-ADP-D-ribose at pH 7.3 and 23°C Escherichia coli
1.84
-
 O-acetyl-ADP-D-ribose at pH 7.3 and 23°C Staphylococcus aureus