Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is intrinsically resistant to beta-lactam inactivation | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43048 | - |
gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(4-D-Ala)-(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + H2O | Staphylococcus aureus | - |
[(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + D-alanine | - |
? | |
[(4-D-Ala)-(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + H2O | Staphylococcus aureus Mu50 | - |
[(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + D-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus Mu50 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
SP-Sepharose column chromatography, Hiprep butyl column chromatography, and Superdex 75 gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(4-D-Ala)-(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + H2O | - |
Staphylococcus aureus | [(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + D-alanine | - |
? | |
[(4-D-Ala)-(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + H2O | - |
Staphylococcus aureus Mu50 | [(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + D-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43031, electrospray ionization mass spectrometry | Staphylococcus aureus |
monomer | 1 * 43032, calculated from amino acid sequence | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
fmtA | - |
Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
physiological function | the penicillin-binding protein may compensate for suppressed peptidoglycan biosynthesis underbeta-lactam induced cell wall stress conditions | Staphylococcus aureus |