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Literature summary for 3.1.1.103 extracted from

  • Fan, X.; Liu, Y.; Smith, D.; Konermann, L.; Siu, K.; Golemi-Kotra, D.
    Diversity of penicillin-binding proteins Resistance factor FmtA of Staphylococcus aureus (2007), J. Biol. Chem., 282, 35143-35152 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is intrinsically resistant to beta-lactam inactivation Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43048
-
gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(4-D-Ala)-(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + H2O Staphylococcus aureus
-
[(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + D-alanine
-
?
[(4-D-Ala)-(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + H2O Staphylococcus aureus Mu50
-
[(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + D-alanine
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus aureus Mu50
-
-
-

Purification (Commentary)

Purification (Comment) Organism
SP-Sepharose column chromatography, Hiprep butyl column chromatography, and Superdex 75 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[(4-D-Ala)-(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + H2O
-
Staphylococcus aureus [(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + D-alanine
-
?
[(4-D-Ala)-(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + H2O
-
Staphylococcus aureus Mu50 [(2-GlcNAc)-Rib-ol-P]n-[Gro-P]m-ManNAc-GlcNAc-PP-peptidoglycan + D-alanine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43031, electrospray ionization mass spectrometry Staphylococcus aureus
monomer 1 * 43032, calculated from amino acid sequence Staphylococcus aureus

Synonyms

Synonyms Comment Organism
fmtA
-
Staphylococcus aureus

General Information

General Information Comment Organism
physiological function the penicillin-binding protein may compensate for suppressed peptidoglycan biosynthesis underbeta-lactam induced cell wall stress conditions Staphylococcus aureus