A bacterium that degrades and assimilates poly(ethylene terephthalate)
Yoshida, S.; Hiraga, K.; Takehana, T.; Taniguchi, I.; Yamaji, H.; Maeda, Y.; Toyohara, K.; Miyamoto, K.; Kimura, Y.; Oda, K.; Science 351, 1196-1199 (2016) 
Data extracted from this reference:
Application
Application
Commentary
Organism
degradation
enzyme shows good activity against commercial bottle-derived PET, which is highly crystallized and is considerably active against PET film at low temperatures
Ideonella sakaiensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0073
-
ethylene terephthalate
pH 7.0, 30°C
Ideonella sakaiensis
Organism
Organism
UniProt
Commentary
Textmining
Ideonella sakaiensis
A0A0K8P8E7
-
-
Ideonella sakaiensis 201-F6
A0A0K8P8E7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ethylene terephthalate + H2O
-
739707
Ideonella sakaiensis
terephthalate + ethylene glycol
-
-
-
?
ethylene terephthalate + H2O
-
739707
Ideonella sakaiensis 201-F6
terephthalate + ethylene glycol
-
-
-
?
additional information
no substrates: poly(ethylene terephthalate), bis(2-hydroxyethyl) terephthalic acid or 4-nitrophenyl aliphatic esters
739707
Ideonella sakaiensis
?
-
-
-
?
additional information
no substrates: poly(ethylene terephthalate), bis(2-hydroxyethyl) terephthalic acid or 4-nitrophenyl aliphatic esters
739707
Ideonella sakaiensis 201-F6
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
ISF6_0224
-
Ideonella sakaiensis
MHETase
-
Ideonella sakaiensis
mono(2-hydroxyethyl) terephthalate hydrolase
-
Ideonella sakaiensis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
31
-
ethylene terephthalate
pH 7.0, 30°C
Ideonella sakaiensis
Application (protein specific)
Application
Commentary
Organism
degradation
enzyme shows good activity against commercial bottle-derived PET, which is highly crystallized and is considerably active against PET film at low temperatures
Ideonella sakaiensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0073
-
ethylene terephthalate
pH 7.0, 30°C
Ideonella sakaiensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ethylene terephthalate + H2O
-
739707
Ideonella sakaiensis
terephthalate + ethylene glycol
-
-
-
?
ethylene terephthalate + H2O
-
739707
Ideonella sakaiensis 201-F6
terephthalate + ethylene glycol
-
-
-
?
additional information
no substrates: poly(ethylene terephthalate), bis(2-hydroxyethyl) terephthalic acid or 4-nitrophenyl aliphatic esters
739707
Ideonella sakaiensis
?
-
-
-
?
additional information
no substrates: poly(ethylene terephthalate), bis(2-hydroxyethyl) terephthalic acid or 4-nitrophenyl aliphatic esters
739707
Ideonella sakaiensis 201-F6
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
31
-
ethylene terephthalate
pH 7.0, 30°C
Ideonella sakaiensis
Other publictions for EC 3.1.1.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739707
Yoshida
A bacterium that degrades and ...
Ideonella sakaiensis, Ideonella sakaiensis 201-F6
Science
351
1196-1199
2016
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737630
Barth
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Effect of hydrolysis products ...
Thermobifida fusca
Biochem. Eng. J.
93
222-228
2015
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