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Literature summary for 3.1.1.102 extracted from

  • Yoshida, S.; Hiraga, K.; Takehana, T.; Taniguchi, I.; Yamaji, H.; Maeda, Y.; Toyohara, K.; Miyamoto, K.; Kimura, Y.; Oda, K.
    A bacterium that degrades and assimilates poly(ethylene terephthalate) (2016), Science, 351, 1196-1199.
    View publication on PubMed

Application

Application Comment Organism
degradation enzyme shows good activity against commercial bottle-derived PET, which is highly crystallized and is considerably active against PET film at low temperatures Ideonella sakaiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0073
-
ethylene terephthalate pH 7.0, 30°C Ideonella sakaiensis

Organism

Organism UniProt Comment Textmining
Ideonella sakaiensis A0A0K8P8E7
-
-
Ideonella sakaiensis 201-F6 A0A0K8P8E7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethylene terephthalate + H2O
-
Ideonella sakaiensis terephthalate + ethylene glycol
-
?
ethylene terephthalate + H2O
-
Ideonella sakaiensis 201-F6 terephthalate + ethylene glycol
-
?
additional information no substrates: poly(ethylene terephthalate), bis(2-hydroxyethyl) terephthalic acid or 4-nitrophenyl aliphatic esters Ideonella sakaiensis ?
-
?
additional information no substrates: poly(ethylene terephthalate), bis(2-hydroxyethyl) terephthalic acid or 4-nitrophenyl aliphatic esters Ideonella sakaiensis 201-F6 ?
-
?

Synonyms

Synonyms Comment Organism
ISF6_0224
-
Ideonella sakaiensis
MHETase
-
Ideonella sakaiensis
mono(2-hydroxyethyl) terephthalate hydrolase
-
Ideonella sakaiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31
-
ethylene terephthalate pH 7.0, 30°C Ideonella sakaiensis