Literature summary for 3.1.1.101 extracted from

Han, X.; Liu, W.; Huang, J.W.; Ma, J.; Zheng, Y.; Ko, T.P.; Xu, L.; Cheng, Y.S.; Chen, C.C.; Guo, R.T.
Structural insight into catalytic mechanism of PET hydrolase (2017), Nat. Commun., 8, 2106 .

Search for more literature for 3.1.1.101

Application

Application	Comment	Organism
degradation	bioconversion of plastics	Ideonella sakaiensis
environmental protection	bioconversion of plastics	Ideonella sakaiensis

Cloned(Commentary)

Cloned (Comment)	Organism
the PETase from Ideonella sakaiensis without the N-terminal 29 amino acids is chemically synthesized, ligated into the pET32a vector, and expressed in Escherichia coli	Ideonella sakaiensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting-drop vapor-diffusion method at 25°C, crystallized in the orthorhombic space group P212121. The structure is solved at 1.58 A resolution	Ideonella sakaiensis

Protein Variants

Protein Variants	Comment	Organism
C174S	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is reduced to less than 5% compared to wild-type value	Ideonella sakaiensis
C210S	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is is abolished	Ideonella sakaiensis
I179A	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is reduced to about 15% compared to wild-type value	Ideonella sakaiensis
M132A	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is reduced to about 15% compared to wild-type value	Ideonella sakaiensis
S131A	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is reduced to less than 5% compared to wild-type value	Ideonella sakaiensis
S185H	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is reduced to about 45% compared to wild-type value	Ideonella sakaiensis
T59A	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is reduced to about 95% compared to wild-type value	Ideonella sakaiensis
W130A	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is reduced to about 15% compared to wild-type value	Ideonella sakaiensis
W130H	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is reduced to about 10% compared to wild-type value	Ideonella sakaiensis
W156A	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is reduced to about 10% compared to wild-type value	Ideonella sakaiensis
Y58A	production of 4-[(2-hydroxyethoxy)carbonyl]benzoate is reduced to about 80% compared towild-type value	Ideonella sakaiensis

Organism

Organism	UniProt	Comment	Textmining
Ideonella sakaiensis	A0A0K8P6T7	-	-
Ideonella sakaiensis 201-F6	A0A0K8P6T7	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Ideonella sakaiensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(ethylene terephthalate)n + H2O	-	Ideonella sakaiensis	(ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate	-	?
(ethylene terephthalate)n + H2O	-	Ideonella sakaiensis 201-F6	(ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Ideonella sakaiensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	assay at	Ideonella sakaiensis

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Release 2023.1 (January 2023)